Induction of antigen-specific systemic and mucosal immune responses by feeding animals transgenic plants expressing the antigen

Jong Lye Bae, Jong Gul Lee, Tae Jin Kang, Hyon Seok Jang, Yong Suk Jang, Moon Sik Yang

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A report from that the presence of lactogenic immunity in pigs protected suckling piglets from porcine epidemic diarrhea virus (PEDV) infection suggested that inducing mucosal immune responses in lactating pigs is an effective way of protecting swine from PEDV infection. In this study, we developed transgenic tobacco plants that express the antigen protein corresponding to the neutralizing epitope of PEDV spike protein, and tested whether feeding the plants to pigs induced an effective immune response against PEDV infection. First, we confirmed the immunogenicity of the plant-derived antigen by using a plaque reduction neutralization assay with serum obtained after injecting mice with protein extracted from the transgenic plants. Feeding the transgenic plants to mice induced both systemic and mucosal immune responses against the antigen. The induced antibodies inhibited virus infection in the plaque reduction neutralization assay. These results suggest that feeding animals transgenic plants carrying antigen genes is an effective strategy to induce protective immune responses against PEDV infection.

Original languageEnglish
Pages (from-to)4052-4058
Number of pages7
JournalVaccine
Volume21
Issue number25-26
DOIs
Publication statusPublished - 2003 Jan 1

Fingerprint

Plant Antigens
Porcine epidemic diarrhea virus
mucosal immunity
Mucosal Immunity
Genetically Modified Plants
animal feeding
Virus Diseases
transgenic plants
antigens
Antigens
Swine
swine
infection
immune response
neutralization tests
Proteins
proteins
Histocompatibility Antigens Class II
mice
suckling

Keywords

  • Edible vaccine
  • Plant
  • Porcine epidemic diarrhea virus

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Induction of antigen-specific systemic and mucosal immune responses by feeding animals transgenic plants expressing the antigen. / Bae, Jong Lye; Lee, Jong Gul; Kang, Tae Jin; Jang, Hyon Seok; Jang, Yong Suk; Yang, Moon Sik.

In: Vaccine, Vol. 21, No. 25-26, 01.01.2003, p. 4052-4058.

Research output: Contribution to journalArticle

Bae, Jong Lye ; Lee, Jong Gul ; Kang, Tae Jin ; Jang, Hyon Seok ; Jang, Yong Suk ; Yang, Moon Sik. / Induction of antigen-specific systemic and mucosal immune responses by feeding animals transgenic plants expressing the antigen. In: Vaccine. 2003 ; Vol. 21, No. 25-26. pp. 4052-4058.
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