Induction of apoptosis in a human lymphoma cell line by hydrophobic peptide fraction separated from anchovy sauce

Gon Lee Young, Won Lee Ki, Yeon Kim Ji, Kyoung Heon Kim, Joo Lee Hyong

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Peptide fractions showing anticancer activity were isolated from anchovy sauce, and their abilities to induce apoptosis in a human lymphoma cell (U937) were determined by biochemical and flow cytometric methods. The butanol extract (fraction Aob) from the desalted hydrophobic peptide fraction (Ao) separated from anchovy sauce by HP-20 adsorption chromatography was found to possess strong antiproliferative activity against U937 by inducing apoptosis. Treatment of U937 with Aob resulted in sub-G1 peak representing apoptosis in the cell cycle analysis, and apoptotic DNA fragmentation. Antiproliferative activity of the peptide fraction further purified successively by silica gel chromatography, TLC, and reversed-phase HPLC increased proportionally with the purification fold. The peptide fraction having antiproliferative activity was found to be composed of Ala and Phe, and its molecular weight was estimated to be 440.9 Da.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalBioFactors
Volume21
Issue number1-4
Publication statusPublished - 2004

Fingerprint

Lymphoma
Cells
Apoptosis
Cell Line
Peptides
Chromatography
U937 Cells
Butanols
Silica Gel
DNA Fragmentation
Adsorption
Purification
Gel Chromatography
Cell Cycle
Molecular Weight
Molecular weight
High Pressure Liquid Chromatography
DNA

Keywords

  • Anchovy sauce
  • Antiproliferative activity
  • Apoptosis
  • Human lymphoma cells
  • Hydrophobic peptide

ASJC Scopus subject areas

  • Biochemistry

Cite this

Induction of apoptosis in a human lymphoma cell line by hydrophobic peptide fraction separated from anchovy sauce. / Young, Gon Lee; Ki, Won Lee; Ji, Yeon Kim; Kim, Kyoung Heon; Hyong, Joo Lee.

In: BioFactors, Vol. 21, No. 1-4, 2004, p. 63-67.

Research output: Contribution to journalArticle

Young, Gon Lee ; Ki, Won Lee ; Ji, Yeon Kim ; Kim, Kyoung Heon ; Hyong, Joo Lee. / Induction of apoptosis in a human lymphoma cell line by hydrophobic peptide fraction separated from anchovy sauce. In: BioFactors. 2004 ; Vol. 21, No. 1-4. pp. 63-67.
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