Induction of CTL responses and identification of a novel epitope of hepatitis B virus surface antigens in C57BL/6 mice immunized with recombinant vaccinia viruses

Sujin Roh, Yun Kyung Lee, Byung Yoon Ahn, Kilhyoun Kiml, Aree Moon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

MHC class I-restricted cytotoxic T lymphocyte (CTL) response to hepatitis B virus (HBV) surface antigens (HBsAg) has been suggested to play essential roles in viral clearance and pathogenesis of HBV-induced hepatitis. In the present study, we analyzed CTL responses to endogenously synthesized or exogenously introduced HBsAg in C57BL/6 mice (H-2b). We show that the endogenously synthesized surface antigens of adr-type HBV encoded by recombinant vaccinia virus efficiently elicit CTL responses in C57BL/6 mice previously defined as non-responders to vaccinia-HBV immunization. We also show that two peptides, S179-186 (FVQWFVGL) and S208-216 (ILSPFLPLL), serve as effective motifs for CTL response in H-2b system after in vitro restimulation of the primed T cells with either of the two synthetic peptides. S208-215 has recently been identified as a CTL epitope which could be produced by exogenous pathway only, in contrast to the current result, while S179-186 appeared a novel epitope for CTL response. In addition, we show that soluble HBsAg also elicits CTL responses in H-2b mice upon in vitro restimulation with the two peptides, although less efficiently compared with the recombinant vaccinia viruses. These findings may provide an efficient experimental system for studying H-2b-restricted immune responses against endogenously synthesized and exogenously introduced HBsAg.

Original languageEnglish
Pages (from-to)17-26
Number of pages10
JournalVirus Research
Volume73
Issue number1
DOIs
Publication statusPublished - 2001

Keywords

  • CTL
  • Epitope peptide
  • H-2
  • Hepatitis B virus
  • Recombinant vaccinia viruses

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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