Induction of Embryoid Body formation using a concave PDMS mold

Yoon Young Choi, Dae Ho Lee, Sang Hoon Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Embryoid Body (EB) is aggregates of embryonic stem cells and differentiation of embryonic stem cells into specific cells for regenerative medicine is often initiated by EB formation. Recent study reported that EB size is important factors both in improving the reproducibility of differentiation and in regulating endogenously influenced cell type-specific differentiation [1]. This paper propose the method to form the uniform sized EBs maintaining their pluripotency using concave microwells.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1231-1233
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09/11/109/11/5

Keywords

  • Embryoid Body (EB)
  • Hanging drop
  • Uniform size EB

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Choi, Y. Y., Lee, D. H., & Lee, S. H. (2009). Induction of Embryoid Body formation using a concave PDMS mold. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1231-1233). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.