Abstract
2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is an environmental toxicant with a polyhalogenated aromatic hydrocarbon structure and is one of the most toxic man-made chemicals. Exposure to 2,3,7,8-TCDD induces reproductive toxicity, immunotoxicity, and hepatotoxicity. In this study, we evaluated how 2,3,7,8-TCDD-induced hepatotoxicity affect the expression of heat shock proteins and antioxidant enzymes using the real-time polymerase chain reaction (PCR) in rat. 2,3,7,8-TCDD increased heat shock protein (Hsp27, α-B-crystallin, Mortalin, Hsp105, and Hsp90s) and antioxidant enzymes (SOD-3, GST and catalase) expression after a 1 day exposure in livers of rats, whereas heat shock protein (α-B-crystallin, Hsp90, and GRP78) and antioxidant enzymes (SOD-1, SOD-3, catalase, GST, and GPXs) expression decreased on day 2 and then slowly recovered back to control levels on day 8. These results suggest that heat shock proteins and antioxidant enzymes were induced as protective mechanisms against 2,3,7,8-TCDD induced hepatotoxicity, and that prolonged exposure depressed their levels, which recovered to control levels due to reduced 2,3,7,8-TCDD induced hepatotoxicity.
| Original language | English |
|---|---|
| Pages (from-to) | 469-476 |
| Number of pages | 8 |
| Journal | Korean Journal of Physiology and Pharmacology |
| Volume | 16 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2012 Dec 1 |
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Keywords
- 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD)
- Antioxidant enzymes
- Gene expression
- Heat shock proteins
- Real-time PCR
ASJC Scopus subject areas
- Physiology
- Pharmacology
Cite this
Induction of heat shock proteins and antioxidant enzymes in 2,3,7,8-tcdd-induced hepatotoxicity in rats. / Kim, Hyun Sook; Park, So Young; Yoo, Ki Yeol; Lee, Seung Kwan; Jung, Woon Won.
In: Korean Journal of Physiology and Pharmacology, Vol. 16, No. 6, 01.12.2012, p. 469-476.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of heat shock proteins and antioxidant enzymes in 2,3,7,8-tcdd-induced hepatotoxicity in rats
AU - Kim, Hyun Sook
AU - Park, So Young
AU - Yoo, Ki Yeol
AU - Lee, Seung Kwan
AU - Jung, Woon Won
PY - 2012/12/1
Y1 - 2012/12/1
N2 - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is an environmental toxicant with a polyhalogenated aromatic hydrocarbon structure and is one of the most toxic man-made chemicals. Exposure to 2,3,7,8-TCDD induces reproductive toxicity, immunotoxicity, and hepatotoxicity. In this study, we evaluated how 2,3,7,8-TCDD-induced hepatotoxicity affect the expression of heat shock proteins and antioxidant enzymes using the real-time polymerase chain reaction (PCR) in rat. 2,3,7,8-TCDD increased heat shock protein (Hsp27, α-B-crystallin, Mortalin, Hsp105, and Hsp90s) and antioxidant enzymes (SOD-3, GST and catalase) expression after a 1 day exposure in livers of rats, whereas heat shock protein (α-B-crystallin, Hsp90, and GRP78) and antioxidant enzymes (SOD-1, SOD-3, catalase, GST, and GPXs) expression decreased on day 2 and then slowly recovered back to control levels on day 8. These results suggest that heat shock proteins and antioxidant enzymes were induced as protective mechanisms against 2,3,7,8-TCDD induced hepatotoxicity, and that prolonged exposure depressed their levels, which recovered to control levels due to reduced 2,3,7,8-TCDD induced hepatotoxicity.
AB - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is an environmental toxicant with a polyhalogenated aromatic hydrocarbon structure and is one of the most toxic man-made chemicals. Exposure to 2,3,7,8-TCDD induces reproductive toxicity, immunotoxicity, and hepatotoxicity. In this study, we evaluated how 2,3,7,8-TCDD-induced hepatotoxicity affect the expression of heat shock proteins and antioxidant enzymes using the real-time polymerase chain reaction (PCR) in rat. 2,3,7,8-TCDD increased heat shock protein (Hsp27, α-B-crystallin, Mortalin, Hsp105, and Hsp90s) and antioxidant enzymes (SOD-3, GST and catalase) expression after a 1 day exposure in livers of rats, whereas heat shock protein (α-B-crystallin, Hsp90, and GRP78) and antioxidant enzymes (SOD-1, SOD-3, catalase, GST, and GPXs) expression decreased on day 2 and then slowly recovered back to control levels on day 8. These results suggest that heat shock proteins and antioxidant enzymes were induced as protective mechanisms against 2,3,7,8-TCDD induced hepatotoxicity, and that prolonged exposure depressed their levels, which recovered to control levels due to reduced 2,3,7,8-TCDD induced hepatotoxicity.
KW - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD)
KW - Antioxidant enzymes
KW - Gene expression
KW - Heat shock proteins
KW - Real-time PCR
UR - http://www.scopus.com/inward/record.url?scp=84876763354&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876763354&partnerID=8YFLogxK
U2 - 10.4196/kjpp.2012.16.6.469
DO - 10.4196/kjpp.2012.16.6.469
M3 - Article
C2 - 23269910
AN - SCOPUS:84876763354
VL - 16
SP - 469
EP - 476
JO - Korean Journal of Physiology and Pharmacology
JF - Korean Journal of Physiology and Pharmacology
SN - 1226-4512
IS - 6
ER -