Induction of heat shock proteins and antioxidant enzymes in 2,3,7,8-tcdd-induced hepatotoxicity in rats

Hyun Sook Kim, So Young Park, Ki Yeol Yoo, Seung Kwan Lee, Woon Won Jung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is an environmental toxicant with a polyhalogenated aromatic hydrocarbon structure and is one of the most toxic man-made chemicals. Exposure to 2,3,7,8-TCDD induces reproductive toxicity, immunotoxicity, and hepatotoxicity. In this study, we evaluated how 2,3,7,8-TCDD-induced hepatotoxicity affect the expression of heat shock proteins and antioxidant enzymes using the real-time polymerase chain reaction (PCR) in rat. 2,3,7,8-TCDD increased heat shock protein (Hsp27, α-B-crystallin, Mortalin, Hsp105, and Hsp90s) and antioxidant enzymes (SOD-3, GST and catalase) expression after a 1 day exposure in livers of rats, whereas heat shock protein (α-B-crystallin, Hsp90, and GRP78) and antioxidant enzymes (SOD-1, SOD-3, catalase, GST, and GPXs) expression decreased on day 2 and then slowly recovered back to control levels on day 8. These results suggest that heat shock proteins and antioxidant enzymes were induced as protective mechanisms against 2,3,7,8-TCDD induced hepatotoxicity, and that prolonged exposure depressed their levels, which recovered to control levels due to reduced 2,3,7,8-TCDD induced hepatotoxicity.

Original languageEnglish
Pages (from-to)469-476
Number of pages8
JournalKorean Journal of Physiology and Pharmacology
Volume16
Issue number6
DOIs
Publication statusPublished - 2012 Dec

Keywords

  • 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD)
  • Antioxidant enzymes
  • Gene expression
  • Heat shock proteins
  • Real-time PCR

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

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