Induction of Id2 expression by cardiac transcription factors GATA4 and Nkx2.5

Joong Yeon Lim, Ho Kim Won, Joon Kim, Ick Park Sang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Inhibitor of differentiation/DNA binding (Id) proteins function as a regulator of helix-loop-helix proteins participating in cell lineage commitment and differentiation. Here, we observed a marked induction of Id2 during cardiomyocyte differentiation from P19CL6 murine embryonic teratocarcinoma stem cells, prompting us to investigate the upstream regulatory mechanism of Id2 induction. Computer analysis of Id2 promoter and subsequent electrophoretic mobility shift assay revealed several binding sites for GATA4 and Nkx2.5 within the Id2 promoter. By further deletion and mutation analysis of the respective binding site, we identified that two motifs located at -497/-502 and -264/-270 were functionally important for Id2 promoter activation by GATA4 and Nkx2.5, respectively. Overexpression of GATA4 and/or Nkx2.5 induced not only Id2 promoter activity but also Id2 protein expression. Additionally, Id proteins significantly inhibit the GATA4 and Nkx2.5-dependent transcription, suggesting Id proteins may play a regulatory role in cardiogenesis. Collectively, our results demonstrate that GATA4 and Nkx2.5 could be one of the upstream regulators of Id2.

Original languageEnglish
Pages (from-to)182-194
Number of pages13
JournalJournal of Cellular Biochemistry
Volume103
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1

Fingerprint

GATA4 Transcription Factor
Proteins
Binding Sites
Embryonal Carcinoma Stem Cells
Electrophoretic mobility
Sequence Deletion
DNA-Binding Proteins
Electrophoretic Mobility Shift Assay
Cell Lineage
Transcription
Embryonic Stem Cells
Stem cells
Cardiac Myocytes
Cell Differentiation
Assays
Chemical activation
DNA

Keywords

  • Cell differentiation
  • Gene expression regulation
  • Idb2 protein
  • Myocytes
  • Transcription factors

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Induction of Id2 expression by cardiac transcription factors GATA4 and Nkx2.5. / Lim, Joong Yeon; Won, Ho Kim; Kim, Joon; Sang, Ick Park.

In: Journal of Cellular Biochemistry, Vol. 103, No. 1, 01.01.2008, p. 182-194.

Research output: Contribution to journalArticle

Lim, Joong Yeon ; Won, Ho Kim ; Kim, Joon ; Sang, Ick Park. / Induction of Id2 expression by cardiac transcription factors GATA4 and Nkx2.5. In: Journal of Cellular Biochemistry. 2008 ; Vol. 103, No. 1. pp. 182-194.
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