Induction of immunity against hepatitis B virus surface antigen by intranasal DNA vaccination using a cationic emulsion as a mucosal gene carrier

Tae Woo Kim, Hesson Chung, Ick Chan Kwon, Ha Chin Sung, Tae Heung Kang, Hee Dong Han, Seo Young Jeong

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Delivery of DNA vaccines to airway mucosa would be an ideal method for mucosal immunization. However, there have been few reports of a suitable gene delivery system. In this study we used a cationic emulsion to immunize mice via the intranasal route with pCMV-S coding for Hepatitis B virus surface antigen (HBsAg). Complexing pCMV-S with a cationic emulsion dramatically enhanced HBsAg expression in both nasal tissue and lung, and was associated with increases in the levels of HBs-specific Abs in serum and mucosal fluids, of cytotoxic T lymphocytes (CTL) in the spleen and cervical and iliac lymph nodes, and of delayed-type hypersensitivity (DTH) against HBsAg. In contrast, very weak humoral and cellular immunities were observed following immunization with naked DNA. In support of these observations, a higher proliferative response of spleenocytes was detected in the group immunized with the emulsion/ pCMV-S complex than in the group immunized with naked pCMV-S. These findings may facilitate development of an emulsion-mediated gene vaccination technique for use against intracellular pathogens that invade mucosal surfaces.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalMolecules and Cells
Volume22
Issue number2
Publication statusPublished - 2006 Oct 1

Keywords

  • Cationic lipid emulsion
  • Hepatitis B virus
  • Mucosal DNA vaccine

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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