Induction of in vivo resistance to Mycobacterium avium infection by intramuscular injection with DNA encoding interleukin-18

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Interferon-gamma (IFN-γ) is closely associated with the generation of cell-mediated immunity and resistance to intracellular parasites. Interleukin-18 (IL-18) was known to strongly induce IFN-γ production by T cells and natural killer (NK) cells. In order to determine whether injection with DNA encoding IL-18 can stimulate the resistance to Mycobacterium avium complex (MAC) infection, the mature IL-18 cDNA with κ leader sequence was cloned under control of the cytomegalovirus (CMV) promoter (TcCMVIL-18) and its effect on MAC infection was investigated in genetically susceptible BALB/c mice. Injection with the TcCMVIL-18 DNA during intranasal infection with MAC resulted in a significant decrease in bacterial load of lung during the entire 8-week observation period, while injection with the TcCMV control DNA did not. Lung cells in mice injected with the TcCMVIL-18 DNA showed persistent production of IFN-γ throughout the 8-week period. Furthermore, immunization with the TcCMVIL-18 DNA induced and maintained significantly higher levels of cytotoxic activity and nitric oxide production by lung cells than immunization with the TcCMV control vector. This work suggests that IL-18 DNA vaccination may be useful in the immunotherapeutic or immunoprotection approaches of infections by intracellular parasites such as mycobacteria.

Original languageEnglish
Pages (from-to)234-241
Number of pages8
JournalImmunology
Volume102
Issue number2
DOIs
Publication statusPublished - 2001 Mar 26
Externally publishedYes

Fingerprint

Mycobacterium avium
Mycobacterium Infections
Interleukin-18
Intramuscular Injections
Mycobacterium avium Complex
DNA
Interferon-gamma
Lung
Injections
Immunization
Infection
Parasitic Diseases
Bacterial Load
Mycobacterium
Cytomegalovirus
Cellular Immunity
Natural Killer Cells
Nitric Oxide
Parasites
Vaccination

ASJC Scopus subject areas

  • Immunology

Cite this

Induction of in vivo resistance to Mycobacterium avium infection by intramuscular injection with DNA encoding interleukin-18. / Kim, S. H.; Cho, Dae Ho; Kim, Tae Sung.

In: Immunology, Vol. 102, No. 2, 26.03.2001, p. 234-241.

Research output: Contribution to journalArticle

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