Induction of kanamycin resistance gene of plasmid pUCD615 by benzoic acid and phenols

Robert J. Mitchell, Han Na Hong, Man Bock Gu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A kan'::luxCDABE fusion strain that was both highly bioluminescent and responsive to benzoic acid was constructed by transforming E. coli strain W3110 with the plasmid pUCDK, which was constructed by digesting and removing the 7-kb KpnI fragment from the promoterless luxCDABE plasmid pUCD615. Experiments using buffered media showed that this induction was dependent on the pH of the media, which influences the degree of benzoic acid protonation, and the expression levels seen are likely due to acidification of the cytoplasm by uncoupling of benzoic acid. Consequently, the sensitivity of this strain for benzoic acid was increased by nearly 20-fold when the pH was shifted from 8.0 to 6.5. Benzoic acid derivatives and several phenolics also resulted in significantly increased bioluminescent signals. Although these compounds are known to damage membranes and induce the heat-shock response within E. coli, bacterial strains harboring mutations in the fadR and rpoH genes, which are responsible for fatty acid biosynthesis during membrane stress and induction of the heat-shock response, respectively, showed that these mutations had no effect on the responses observed.

Original languageEnglish
Pages (from-to)1125-1131
Number of pages7
JournalJournal of microbiology and biotechnology
Volume16
Issue number7
Publication statusPublished - 2006 Jul

Keywords

  • Benzoic acid
  • Bioluminescence
  • Kanamycin
  • Uncoupling
  • acc (6′)-Ib

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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