Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1

Jai Hee Moon, Byung Sun Yoon, Bona Kim, Gyuman Park, Hye Youn Jung, Isaac Maeng, Eun Kyoung Jun, Seung Jun Yoo, Aeree Kim, Sejong Oh, Kwang Youn Whang, Hyunggee Kim, Dong Wook Kim, Ki Dong Kim, Seungkwon You

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1.

Original languageEnglish
Pages (from-to)267-272
Number of pages6
JournalBiochemical and biophysical research communications
Volume371
Issue number2
DOIs
Publication statusPublished - 2008 Jun 27

Keywords

  • Astrocytes
  • Dedifferentiation
  • Neural stem cell-like cells
  • Neural stem cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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