Induction of serum amyloid A genes is associated with growth and apoptosis of HC11 mammary epithelial cells

Yoonjung Kho, Sungchan Kim, Byung Sun Yoon, Jai Hee Moon, Bona Kim, Sungwook Kwak, Junghee Woo, Sejong Oh, Kichang Hong, Saehun Kim, Hyunggee Kim, Seungkwon You, Yunjaie Choi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In this study, we examined the expression and functions of serum amyloid A (SAA) isoforms during apoptosis of HC11 mammary gland epithelial cells. Expression of SAA mRNAs and apoptosis were increased in HC11 cells by serum withdrawal and gradually decreased upon the addition of serum, or epidermal growth factor (EGF). TNFα treatment of HC11 cells also induced expression of SAA genes, and the effect on SAA1 and SAA2 expression was suppressed by treatment with MG132, and in cells transfected with a dominant negative mutant form of IκBα. Similar results were observed in response to interleukin-1 (IL-1), IL-6 and interferon γ (IFNγ). Furthermore, overexpression of the SAA1 and SAA2 isoforms suppressed growth and accelerated apoptosis of HC11 cells by increasing caspase 3/7 and caspase 8 activities, but the apoptotic effect of tumor necrosis factor α (TNFα) on HC11 cells was not enhanced. We found that expression of SAA1 and SAA2, but not SAA3, was regulated by an NFκB-dependent pathway, and that overexpression of SAA isoforms accelerated the apoptosis of HC11 cells.

Original languageEnglish
Pages (from-to)70-81
Number of pages12
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number1
DOIs
Publication statusPublished - 2008

Keywords

  • Apoptosis
  • Cytokines
  • Mammary epithelial cell
  • NFκB
  • Serum amyloid A

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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