Inertial microfluidic cell hydroporator (IMCH) for high-throughput & single-step intracellular molecule delivery

Yanxiang Deng, Miran Rada, Megan Kizer, Xing Wang, Dong Joo Cheon, Aram J. Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a high-throughput and single-step inertial microfluidic cell hydroporator (iMCH) capable of delivering diverse molecules to various cell types with a throughput of >30,000 cells/sec. Our approach is comprised of three sequential processes: (1) Cell injection, where cell suspensions with macromolecules are injected into a microchannel, (2) Inertial cell positioning, where randomly incoming cells are aligned via fluid inertia, and (3) Molecule delivery, where cells deform with large strain upon collision at a T-junction, and the rapid mechanical deformation of cells creates transient membrane openings that facilitate diffusion of molecules into the cytosol.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages169-170
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2020 Jan 1
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 2017 Oct 222017 Oct 26

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period17/10/2217/10/26

Keywords

  • Inertial microfluidic cell hydroporator (iMCH)
  • Inertial microfluidics
  • Intracellular delivery

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Deng, Y., Rada, M., Kizer, M., Wang, X., Cheon, D. J., & Chung, A. J. (2020). Inertial microfluidic cell hydroporator (IMCH) for high-throughput & single-step intracellular molecule delivery. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 169-170). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.