Inflammation by activated macrophage-like THP-1 cells increases human dura mater cell adhesion with alteration of integrin α 2 β 1 and matrix metalloproteinase

Kyuha Chong, Woo Keun Kwon, Joo Han Kim, Youn Kwan Park, Wonki Yoon, Jong Hyun Kim, Taek Hyun Kwon, Hong Joo Moon

Research output: Contribution to journalArticlepeer-review


This study was designed to investigate (i) extracellular matrix to specify adhesive substrates to human dura mater cell (hDMC); (ii) the alteration on adhesion-related molecules in hDMC; and (iii) secreted matrix metalloproteinases (MMPs) linked with extracellular matrix remodeling after exposure to inflammation. The hDMC was cultured from human dura mater tissue, and the studies were performed with hDMC after co-culturing with macrophage like THP-1 cells (Mϕ). The adhesion of co-cultured hDMC through collagen I increased 6.4-fold and through collagen IV increased 5.0-fold compared with the adhesion of naïve cells (p < 0.001). Integrin subtype α 2 β 1 expression was increased 6.3-fold (p < 0.001) and α 1 expression was decreased 2.0-fold (p < 0.001) in the co-cultured cells compared with the naïve cells. Co-culturing induced significant increases in MMP-1 (13.9-fold, p < 0.01), MMP-3 (7.6-fold, p < 0.01), and VEGF (VEGF: 3.8-fold, p < 0.05) expression and decreases in MMP-9 (0.1-fold, p < 0.01) compared with the sum of naïve hDMC and Mϕ values. Increased hDMC adhesion under inflammatory conditions is caused by an increased cellular affinity for collagen I and IV mediated by increased hDMC levels of integrin subtype α 2 β 1 and environmental MMP-1, -3 and decreased MMP-9. Selective integrin subtype α 2 β 1 inhibition assay showed 37.8% and 35.7% reduction in adhesion of co-cultured hDMC to collagen I (p < 0.001) and IV (p = 0.057), respectively. The present study provides insight into the pathological conditions related to dura mater adhesion in inflammation.

Original languageEnglish
Pages (from-to)706-716
Number of pages11
JournalJournal of Orthopaedic Research
Issue number3
Publication statusPublished - 2019 Mar


  • cell adhesion
  • dura mater
  • extracellular matrix
  • inflammation
  • integrin
  • matrix metalloproteinase

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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