Influence of polymorphism of GSTM1 gene on association between glycophorin a mutant frequency and urinary PAH metabolites in incineration workers

Kyoung Ho Lee, Jeongmi Lee, Mina Ha, Jae Wook Choi, Soo Hun Cho, Eung Soo Hwang, Chung Gyu Park, Paul T. Strickland, Ari Hirvonen, Daehee Kang

Research output: Contribution to journalArticle

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Abstract

The association between urinary 1-hydroxypyrene glucuronide (1-OHPG) levels, as an internal measure of polycyclic aromatic hydrocarbon (PAH) exposure, and glycophorin A (GPA) mutation frequency, as an early biologic effect indicator, was determined to establish whether genetic polymorphisms of glutathione S-transferase (GST) isoforms GSTM1 and GSTT1 play a role. Eighty-one workers including 38 employees directly involved in incinerating industry wastes were recruited from a company located in South Korea. Urinary 1-OHPG levels were measured by synchronous fluorescence spectroscopy after immunoaffinity purification using monoclonal antibody 8E11. Erythrocyte GPA variant frequency (NN or NO) was assessed in MN heterozygotes with a flow cytometric assay. The GSTM1 and GSTT1 genotypes were assessed by a multiplex polymerase chain reaction (PCR) method. Urinary 1-OHPG levels were higher in workers handling industrial wastes than in those with presumed lower exposure to PAHs. An increase was seen in GPA variant frequency levels with increase in urinary 1-OHPG levels. When this association was evaluated by GSTM1 genotype status, the association between GPA mutation and urinary 1-OHPG levels was stronger in individuals with GSTM1 genotype. These results suggest that the association between urinary 1-OHPG and GPA mutation might be modulated by the GSTM1 genotype.

Original languageEnglish
Pages (from-to)355-363
Number of pages9
JournalJournal of Toxicology and Environmental Health - Part A
Volume65
Issue number5-6
DOIs
Publication statusPublished - 2002 Apr 10

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Incineration
Glycophorin
Polycyclic Aromatic Hydrocarbons
Metabolites
Polymorphism
Genes
Association reactions
Genotype
Industrial Waste
Republic of Korea
Mutation
Multiplex Polymerase Chain Reaction
Polymerase chain reaction
Fluorescence Spectrometry
Fluorescence spectroscopy
Genetic Polymorphisms
Mutation Rate
Heterozygote
Polycyclic aromatic hydrocarbons
Glutathione Transferase

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Influence of polymorphism of GSTM1 gene on association between glycophorin a mutant frequency and urinary PAH metabolites in incineration workers. / Lee, Kyoung Ho; Lee, Jeongmi; Ha, Mina; Choi, Jae Wook; Cho, Soo Hun; Hwang, Eung Soo; Park, Chung Gyu; Strickland, Paul T.; Hirvonen, Ari; Kang, Daehee.

In: Journal of Toxicology and Environmental Health - Part A, Vol. 65, No. 5-6, 10.04.2002, p. 355-363.

Research output: Contribution to journalArticle

Lee, Kyoung Ho ; Lee, Jeongmi ; Ha, Mina ; Choi, Jae Wook ; Cho, Soo Hun ; Hwang, Eung Soo ; Park, Chung Gyu ; Strickland, Paul T. ; Hirvonen, Ari ; Kang, Daehee. / Influence of polymorphism of GSTM1 gene on association between glycophorin a mutant frequency and urinary PAH metabolites in incineration workers. In: Journal of Toxicology and Environmental Health - Part A. 2002 ; Vol. 65, No. 5-6. pp. 355-363.
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