Influence of wavefront dynamics on transmembrane potential characteristics during atrial fibrillation

Charles A. Athill, Tsu Juey Wu, Masaaki Yashima, Young Hoon Kim, James N. Weiss, Hsun Lun A. Huang, Hrayr S. Karagueuzian, Peng Sheng Chen

Research output: Contribution to journalArticle

Abstract

Transmembrane Potential Characteristics. Introduction: Although computerized mapping studies have demonstrated the presence of multiple wavelets during atrial fibrillation (AF) and that action potential amplitude and duration in AF vary significantly from beat to beat, no study has correlated the single cell action potential changes with the patterns of activation during AF. Methods and Results: We studied wavefront dynamics and single cell transmembrane potential (TMP) characteristics in 12 isolated perfused canine right atria. The endocardial surface was mapped using 477 bipolar electrodes while TMP was recorded with a standard glass microelectrode from an epicardial cell. AF was induced in the presence of acetylcholine. Successful simultaneous TMP recordings and activation maps were made during six episodes of AF and for a total of 141 activations. Large variations of TMP amplitude and duration were observed frequently; 34% of them have a low amplitude (<50% of the amplitude recorded during pacing). Low-amplitude potentials were recorded when the impaled cell was (1) in an area of random reentry (67%, n = 36); (2) within 3.2 mm of the core of organized functional reentry (22%, n = 12); (3) in the middle of two merging wavefronts (9%, n = 5); and (4) at the point of spontaneous wavebreak (2%, n = 1). Conclusion: Large variations of TMP are observed frequently during in vitro AF. Low-amplitude TMPs are associated with specific patterns of AF activation wavefronts.

Original languageEnglish
Pages (from-to)913-921
Number of pages9
JournalJournal of Cardiovascular Electrophysiology
Volume11
Issue number8
Publication statusPublished - 2000 Sep 12
Externally publishedYes

Fingerprint

Membrane Potentials
Atrial Fibrillation
Action Potentials
Thymidine Monophosphate
Microelectrodes
Heart Atria
Acetylcholine
Glass
Canidae
Electrodes

Keywords

  • Action potential duration
  • Reentry
  • Repolarization
  • Wavefront

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Athill, C. A., Wu, T. J., Yashima, M., Kim, Y. H., Weiss, J. N., Huang, H. L. A., ... Chen, P. S. (2000). Influence of wavefront dynamics on transmembrane potential characteristics during atrial fibrillation. Journal of Cardiovascular Electrophysiology, 11(8), 913-921.

Influence of wavefront dynamics on transmembrane potential characteristics during atrial fibrillation. / Athill, Charles A.; Wu, Tsu Juey; Yashima, Masaaki; Kim, Young Hoon; Weiss, James N.; Huang, Hsun Lun A.; Karagueuzian, Hrayr S.; Chen, Peng Sheng.

In: Journal of Cardiovascular Electrophysiology, Vol. 11, No. 8, 12.09.2000, p. 913-921.

Research output: Contribution to journalArticle

Athill, CA, Wu, TJ, Yashima, M, Kim, YH, Weiss, JN, Huang, HLA, Karagueuzian, HS & Chen, PS 2000, 'Influence of wavefront dynamics on transmembrane potential characteristics during atrial fibrillation', Journal of Cardiovascular Electrophysiology, vol. 11, no. 8, pp. 913-921.
Athill, Charles A. ; Wu, Tsu Juey ; Yashima, Masaaki ; Kim, Young Hoon ; Weiss, James N. ; Huang, Hsun Lun A. ; Karagueuzian, Hrayr S. ; Chen, Peng Sheng. / Influence of wavefront dynamics on transmembrane potential characteristics during atrial fibrillation. In: Journal of Cardiovascular Electrophysiology. 2000 ; Vol. 11, No. 8. pp. 913-921.
@article{ae57a43547a3485b970c07090b2363eb,
title = "Influence of wavefront dynamics on transmembrane potential characteristics during atrial fibrillation",
abstract = "Transmembrane Potential Characteristics. Introduction: Although computerized mapping studies have demonstrated the presence of multiple wavelets during atrial fibrillation (AF) and that action potential amplitude and duration in AF vary significantly from beat to beat, no study has correlated the single cell action potential changes with the patterns of activation during AF. Methods and Results: We studied wavefront dynamics and single cell transmembrane potential (TMP) characteristics in 12 isolated perfused canine right atria. The endocardial surface was mapped using 477 bipolar electrodes while TMP was recorded with a standard glass microelectrode from an epicardial cell. AF was induced in the presence of acetylcholine. Successful simultaneous TMP recordings and activation maps were made during six episodes of AF and for a total of 141 activations. Large variations of TMP amplitude and duration were observed frequently; 34{\%} of them have a low amplitude (<50{\%} of the amplitude recorded during pacing). Low-amplitude potentials were recorded when the impaled cell was (1) in an area of random reentry (67{\%}, n = 36); (2) within 3.2 mm of the core of organized functional reentry (22{\%}, n = 12); (3) in the middle of two merging wavefronts (9{\%}, n = 5); and (4) at the point of spontaneous wavebreak (2{\%}, n = 1). Conclusion: Large variations of TMP are observed frequently during in vitro AF. Low-amplitude TMPs are associated with specific patterns of AF activation wavefronts.",
keywords = "Action potential duration, Reentry, Repolarization, Wavefront",
author = "Athill, {Charles A.} and Wu, {Tsu Juey} and Masaaki Yashima and Kim, {Young Hoon} and Weiss, {James N.} and Huang, {Hsun Lun A.} and Karagueuzian, {Hrayr S.} and Chen, {Peng Sheng}",
year = "2000",
month = "9",
day = "12",
language = "English",
volume = "11",
pages = "913--921",
journal = "Journal of Cardiovascular Electrophysiology",
issn = "1045-3873",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Influence of wavefront dynamics on transmembrane potential characteristics during atrial fibrillation

AU - Athill, Charles A.

AU - Wu, Tsu Juey

AU - Yashima, Masaaki

AU - Kim, Young Hoon

AU - Weiss, James N.

AU - Huang, Hsun Lun A.

AU - Karagueuzian, Hrayr S.

AU - Chen, Peng Sheng

PY - 2000/9/12

Y1 - 2000/9/12

N2 - Transmembrane Potential Characteristics. Introduction: Although computerized mapping studies have demonstrated the presence of multiple wavelets during atrial fibrillation (AF) and that action potential amplitude and duration in AF vary significantly from beat to beat, no study has correlated the single cell action potential changes with the patterns of activation during AF. Methods and Results: We studied wavefront dynamics and single cell transmembrane potential (TMP) characteristics in 12 isolated perfused canine right atria. The endocardial surface was mapped using 477 bipolar electrodes while TMP was recorded with a standard glass microelectrode from an epicardial cell. AF was induced in the presence of acetylcholine. Successful simultaneous TMP recordings and activation maps were made during six episodes of AF and for a total of 141 activations. Large variations of TMP amplitude and duration were observed frequently; 34% of them have a low amplitude (<50% of the amplitude recorded during pacing). Low-amplitude potentials were recorded when the impaled cell was (1) in an area of random reentry (67%, n = 36); (2) within 3.2 mm of the core of organized functional reentry (22%, n = 12); (3) in the middle of two merging wavefronts (9%, n = 5); and (4) at the point of spontaneous wavebreak (2%, n = 1). Conclusion: Large variations of TMP are observed frequently during in vitro AF. Low-amplitude TMPs are associated with specific patterns of AF activation wavefronts.

AB - Transmembrane Potential Characteristics. Introduction: Although computerized mapping studies have demonstrated the presence of multiple wavelets during atrial fibrillation (AF) and that action potential amplitude and duration in AF vary significantly from beat to beat, no study has correlated the single cell action potential changes with the patterns of activation during AF. Methods and Results: We studied wavefront dynamics and single cell transmembrane potential (TMP) characteristics in 12 isolated perfused canine right atria. The endocardial surface was mapped using 477 bipolar electrodes while TMP was recorded with a standard glass microelectrode from an epicardial cell. AF was induced in the presence of acetylcholine. Successful simultaneous TMP recordings and activation maps were made during six episodes of AF and for a total of 141 activations. Large variations of TMP amplitude and duration were observed frequently; 34% of them have a low amplitude (<50% of the amplitude recorded during pacing). Low-amplitude potentials were recorded when the impaled cell was (1) in an area of random reentry (67%, n = 36); (2) within 3.2 mm of the core of organized functional reentry (22%, n = 12); (3) in the middle of two merging wavefronts (9%, n = 5); and (4) at the point of spontaneous wavebreak (2%, n = 1). Conclusion: Large variations of TMP are observed frequently during in vitro AF. Low-amplitude TMPs are associated with specific patterns of AF activation wavefronts.

KW - Action potential duration

KW - Reentry

KW - Repolarization

KW - Wavefront

UR - http://www.scopus.com/inward/record.url?scp=0033847881&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033847881&partnerID=8YFLogxK

M3 - Article

C2 - 10969755

AN - SCOPUS:0033847881

VL - 11

SP - 913

EP - 921

JO - Journal of Cardiovascular Electrophysiology

JF - Journal of Cardiovascular Electrophysiology

SN - 1045-3873

IS - 8

ER -