Inhibition of biofilm formation on ventilation tubes by surface modification

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Aim: The purpose of this study was to modify the surface characteristics of a ventilation tube (VT) with polyethylene glycol (PEG) coating and to evaluate the effect on biofilm formation. Materials and Methods: VTs made of polyethylene were coated with PEG. Streptococcus pneumonia R6 strain was used and a crystal violet assay was carried out to measure the in vitro and in vivo biofilm formation of rats bearing VTs. Results: In the in vitro experiment, the optical density of the uncoated VT was 0.34±0.09 and the optical density of the PEGgrafted VT was 0.22±0.06 (p<0.05). In the in vivo experiment, the optical density of the uncoated VT was 0.54±0.12 and that of the PEG-grafted VT was 0.32±0.13 (p<0.05). Scanning electron microscopy showed that surface modification, roughness and hydrophilic characteristics improved and biofilm formation decreased. Conclusion: The reduced biofilm formation on the VT may be explained by the alteration of surface tension and roughness induced by PEG coating.

Original languageEnglish
Pages (from-to)907-912
Number of pages6
JournalIn Vivo
Issue number6
Publication statusPublished - 2012 Nov 1



  • Biofilm
  • PEG coating
  • Streptococcus pneumoniae R6
  • Ventilation tube

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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