Inhibition of biofilm formation on ventilation tubes by surface modification

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aim: The purpose of this study was to modify the surface characteristics of a ventilation tube (VT) with polyethylene glycol (PEG) coating and to evaluate the effect on biofilm formation. Materials and Methods: VTs made of polyethylene were coated with PEG. Streptococcus pneumonia R6 strain was used and a crystal violet assay was carried out to measure the in vitro and in vivo biofilm formation of rats bearing VTs. Results: In the in vitro experiment, the optical density of the uncoated VT was 0.34±0.09 and the optical density of the PEGgrafted VT was 0.22±0.06 (p<0.05). In the in vivo experiment, the optical density of the uncoated VT was 0.54±0.12 and that of the PEG-grafted VT was 0.32±0.13 (p<0.05). Scanning electron microscopy showed that surface modification, roughness and hydrophilic characteristics improved and biofilm formation decreased. Conclusion: The reduced biofilm formation on the VT may be explained by the alteration of surface tension and roughness induced by PEG coating.

Original languageEnglish
Pages (from-to)907-912
Number of pages6
JournalIn Vivo
Volume26
Issue number6
Publication statusPublished - 2012 Nov 1

Fingerprint

Biofilms
Ventilation
Surface treatment
Density (optical)
Bearings (structural)
Surface roughness
Gentian Violet
Coatings
Surface Tension
Polyethylene
Streptococcus pneumoniae
Electron Scanning Microscopy
Surface tension
Rats
Assays
Experiments
Scanning electron microscopy

Keywords

  • Biofilm
  • PEG coating
  • Streptococcus pneumoniae R6
  • Ventilation tube

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Inhibition of biofilm formation on ventilation tubes by surface modification. / Song, Jae-Jun; Noh, Insup; Chae, Sungwon.

In: In Vivo, Vol. 26, No. 6, 01.11.2012, p. 907-912.

Research output: Contribution to journalArticle

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N2 - Aim: The purpose of this study was to modify the surface characteristics of a ventilation tube (VT) with polyethylene glycol (PEG) coating and to evaluate the effect on biofilm formation. Materials and Methods: VTs made of polyethylene were coated with PEG. Streptococcus pneumonia R6 strain was used and a crystal violet assay was carried out to measure the in vitro and in vivo biofilm formation of rats bearing VTs. Results: In the in vitro experiment, the optical density of the uncoated VT was 0.34±0.09 and the optical density of the PEGgrafted VT was 0.22±0.06 (p<0.05). In the in vivo experiment, the optical density of the uncoated VT was 0.54±0.12 and that of the PEG-grafted VT was 0.32±0.13 (p<0.05). Scanning electron microscopy showed that surface modification, roughness and hydrophilic characteristics improved and biofilm formation decreased. Conclusion: The reduced biofilm formation on the VT may be explained by the alteration of surface tension and roughness induced by PEG coating.

AB - Aim: The purpose of this study was to modify the surface characteristics of a ventilation tube (VT) with polyethylene glycol (PEG) coating and to evaluate the effect on biofilm formation. Materials and Methods: VTs made of polyethylene were coated with PEG. Streptococcus pneumonia R6 strain was used and a crystal violet assay was carried out to measure the in vitro and in vivo biofilm formation of rats bearing VTs. Results: In the in vitro experiment, the optical density of the uncoated VT was 0.34±0.09 and the optical density of the PEGgrafted VT was 0.22±0.06 (p<0.05). In the in vivo experiment, the optical density of the uncoated VT was 0.54±0.12 and that of the PEG-grafted VT was 0.32±0.13 (p<0.05). Scanning electron microscopy showed that surface modification, roughness and hydrophilic characteristics improved and biofilm formation decreased. Conclusion: The reduced biofilm formation on the VT may be explained by the alteration of surface tension and roughness induced by PEG coating.

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