Inhibition of c-Yes induces differentiation of HT-29 human colon cancer stem cells through midbody elongation

Jessica Jung, Sung Chul Choi, Han Na Lee, Gi Yeon Han, Chan Wha Kim

Research output: Contribution to journalArticle

Abstract

Recent research suggests that a small group of cells, named cancer stem cells (CSCs), is responsible for initiating tumor formation, recurrence, and metastasis. c-Yes, a proto-oncogene that is a subfamily of Src family kinase, is often activated in human colon cancer; this implicates c-Yes in the onset and progression of the disease. The objective of this study was to investigate the correlation between c-Yes and CSCs. We performed a sphere formation assay and reverse transcription-polymerase chain reaction for studying the differentiation of HT-29 human colon CSCs. To demonstrate the specific role of c-Yes in CSCs, we performed live cell microscopy and a cell cycle assay. These study shows, for the first time, that c-Yes is enriched in CD133+ CSCs, compared to their CD133 counterparts, and that c-Yes depletion in CD133+ cells induces cell differentiation. Moreover, c-Yes depletion was found to elongate the midbody and increase the proliferation doubling time. This also suggested that the misregulation of microtubules during chromosomal separation causes aneuploidy. Our results suggest that c-Yes may play a crucial role in initiating, maintaining, and driving the tumorigenic property of colon cancer.

Original languageEnglish
Pages (from-to)261-269
Number of pages9
JournalTissue Engineering and Regenerative Medicine
Volume13
Issue number3
DOIs
Publication statusPublished - 2016 Jun 1

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Keywords

  • c-Yes
  • Cancer stem cell
  • Cell cycle
  • Differentiation
  • Src family kinase

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

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