TY - JOUR
T1 - Inhibition of c-Yes induces differentiation of HT-29 human colon cancer stem cells through midbody elongation
AU - Jung, Jessica
AU - Choi, Sung Chul
AU - Lee, Han Na
AU - Han, Gi Yeon
AU - Kim, Chan Wha
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Recent research suggests that a small group of cells, named cancer stem cells (CSCs), is responsible for initiating tumor formation, recurrence, and metastasis. c-Yes, a proto-oncogene that is a subfamily of Src family kinase, is often activated in human colon cancer; this implicates c-Yes in the onset and progression of the disease. The objective of this study was to investigate the correlation between c-Yes and CSCs. We performed a sphere formation assay and reverse transcription-polymerase chain reaction for studying the differentiation of HT-29 human colon CSCs. To demonstrate the specific role of c-Yes in CSCs, we performed live cell microscopy and a cell cycle assay. These study shows, for the first time, that c-Yes is enriched in CD133+ CSCs, compared to their CD133− counterparts, and that c-Yes depletion in CD133+ cells induces cell differentiation. Moreover, c-Yes depletion was found to elongate the midbody and increase the proliferation doubling time. This also suggested that the misregulation of microtubules during chromosomal separation causes aneuploidy. Our results suggest that c-Yes may play a crucial role in initiating, maintaining, and driving the tumorigenic property of colon cancer.
AB - Recent research suggests that a small group of cells, named cancer stem cells (CSCs), is responsible for initiating tumor formation, recurrence, and metastasis. c-Yes, a proto-oncogene that is a subfamily of Src family kinase, is often activated in human colon cancer; this implicates c-Yes in the onset and progression of the disease. The objective of this study was to investigate the correlation between c-Yes and CSCs. We performed a sphere formation assay and reverse transcription-polymerase chain reaction for studying the differentiation of HT-29 human colon CSCs. To demonstrate the specific role of c-Yes in CSCs, we performed live cell microscopy and a cell cycle assay. These study shows, for the first time, that c-Yes is enriched in CD133+ CSCs, compared to their CD133− counterparts, and that c-Yes depletion in CD133+ cells induces cell differentiation. Moreover, c-Yes depletion was found to elongate the midbody and increase the proliferation doubling time. This also suggested that the misregulation of microtubules during chromosomal separation causes aneuploidy. Our results suggest that c-Yes may play a crucial role in initiating, maintaining, and driving the tumorigenic property of colon cancer.
KW - Cancer stem cell
KW - Cell cycle
KW - Differentiation
KW - Src family kinase
KW - c-Yes
UR - http://www.scopus.com/inward/record.url?scp=84973332890&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973332890&partnerID=8YFLogxK
U2 - 10.1007/s13770-016-9053-x
DO - 10.1007/s13770-016-9053-x
M3 - Article
AN - SCOPUS:84973332890
VL - 13
SP - 261
EP - 269
JO - Tissue Engineering and Regenerative Medicine
JF - Tissue Engineering and Regenerative Medicine
SN - 1738-2696
IS - 3
ER -