Phenylpyropenes A, B, and C, isolated from Penicillium griseofulvum F1959, inhibited DGAT in rat liver microsomes with IC50 values of 78.7±1.6, 21.7±0.2, and 11.04±0.2 μM, respectively. In addition, a kinetic analysis using a Lineweaver-Burk plot revealed that phenylpyropene C was a noncompetitive inhibitor of DGAT. The apparent Michaelis constant (Km) value and inhibition constant (Ki) value were calculated to be 8 μM and 10.4 μM, respectively. Moreover, phenylpyropene C inhibited triglyceride formation in HepG2 cells.
- Diacylglycerol acyltransferase (DGAT)
- HepG2 cell
- Penicillium griseofulvum F1959
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology