Inhibition of diacylglycerol acyltransferase by phenylpyropenes produced by Penicillium griseofulvum F1959

Seung Woong Lee, Mun Chual Rho, Jung Ho Choi, Koanhoi Kim, Yong Seok Choi, Hyun Sun Lee, Young Kook Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Phenylpyropenes A, B, and C, isolated from Penicillium griseofulvum F1959, inhibited DGAT in rat liver microsomes with IC50 values of 78.7±1.6, 21.7±0.2, and 11.04±0.2 μM, respectively. In addition, a kinetic analysis using a Lineweaver-Burk plot revealed that phenylpyropene C was a noncompetitive inhibitor of DGAT. The apparent Michaelis constant (Km) value and inhibition constant (Ki) value were calculated to be 8 μM and 10.4 μM, respectively. Moreover, phenylpyropene C inhibited triglyceride formation in HepG2 cells.

Original languageEnglish
Pages (from-to)1785-1788
Number of pages4
JournalJournal of microbiology and biotechnology
Volume18
Issue number11
DOIs
Publication statusPublished - 2008 Nov 28

Keywords

  • Diacylglycerol acyltransferase (DGAT)
  • HepG2 cell
  • Penicillium griseofulvum F1959
  • Phenylpyropene
  • Triglyceride

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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