Inhibition of Escherichia coli O157: H7 on stainless steel using Pseudomonas veronii biofilms

Y. Kim, H. Kim, L. R. Beuchat, Jee-Hoon Ryu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We produced a Pseudomonas veronii biofilm on the surface of a stainless steel that is inhibitory to Escherichia coli O157:H7. Pseudomonas veronii strain KACC 81051BP, isolated from lettuce, readily formed biofilm on the surface of stainless steel coupons (SSCs) immersed in tryptic soy broth at 25°C. Cells showed significantly (P ≤ 0·05) enhanced tolerance to desiccation stress (43% relative humidity (RH)) and retained antimicrobial activity against E. coli O157:H7. The number of E. coli O157:H7 (control; 4·1 ± 0·1 log CFU per coupon) on sterile SSCs decreased to 2·7 ± 0·2 log CFU per coupon after exposure to 43% RH at 25°C for 48 h, while the population of E. coli O157:H7 (4·1 ± 0·0 log CFU per coupon) on SSCs containing P. veronii biofilm decreased to below the theoretical detection limit (1·5 log CFU per coupon) within 24 h. The antimicrobial biofilm produced on stainless steel may have application in preventing cross-contamination by E. coli O157:H7 on other abiotic surfaces in food-contact environments. Significance and Impact of the Study: The presence of Escherichia coli O157:H7 on environmental surfaces of food manufacturing, transportation and storage facilities is a significant food safety concern because it can result in cross-contamination of food products. In this study, we developed a Pseudomonas veronii biofilm on the surface of a stainless steel that inhibits the growth of E. coli O157:H7. Since P. veronii in biofilm resists desiccation, it provides persistent antimicrobial activity. Information presented here provides novel and practical insights to developing biological strategies to inactivate E. coli O157:H7 on diverse surfaces in food processing and handling environments.

Original languageEnglish
Pages (from-to)394-399
Number of pages6
JournalLetters in Applied Microbiology
Volume66
Issue number5
DOIs
Publication statusPublished - 2018 May 1

Fingerprint

Escherichia coli O157
Stainless Steel
Biofilms
Pseudomonas
Desiccation
Food Handling
Humidity
Food Contamination
Food
Lettuce
Food Safety
Limit of Detection
Growth

Keywords

  • antagonistic bacteria
  • biofilm
  • desiccation
  • Escherichia coli O157:H7
  • Pseudomonas veronii
  • stainless steel surface

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Inhibition of Escherichia coli O157 : H7 on stainless steel using Pseudomonas veronii biofilms. / Kim, Y.; Kim, H.; Beuchat, L. R.; Ryu, Jee-Hoon.

In: Letters in Applied Microbiology, Vol. 66, No. 5, 01.05.2018, p. 394-399.

Research output: Contribution to journalArticle

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abstract = "We produced a Pseudomonas veronii biofilm on the surface of a stainless steel that is inhibitory to Escherichia coli O157:H7. Pseudomonas veronii strain KACC 81051BP, isolated from lettuce, readily formed biofilm on the surface of stainless steel coupons (SSCs) immersed in tryptic soy broth at 25°C. Cells showed significantly (P ≤ 0·05) enhanced tolerance to desiccation stress (43{\%} relative humidity (RH)) and retained antimicrobial activity against E. coli O157:H7. The number of E. coli O157:H7 (control; 4·1 ± 0·1 log CFU per coupon) on sterile SSCs decreased to 2·7 ± 0·2 log CFU per coupon after exposure to 43{\%} RH at 25°C for 48 h, while the population of E. coli O157:H7 (4·1 ± 0·0 log CFU per coupon) on SSCs containing P. veronii biofilm decreased to below the theoretical detection limit (1·5 log CFU per coupon) within 24 h. The antimicrobial biofilm produced on stainless steel may have application in preventing cross-contamination by E. coli O157:H7 on other abiotic surfaces in food-contact environments. Significance and Impact of the Study: The presence of Escherichia coli O157:H7 on environmental surfaces of food manufacturing, transportation and storage facilities is a significant food safety concern because it can result in cross-contamination of food products. In this study, we developed a Pseudomonas veronii biofilm on the surface of a stainless steel that inhibits the growth of E. coli O157:H7. Since P. veronii in biofilm resists desiccation, it provides persistent antimicrobial activity. Information presented here provides novel and practical insights to developing biological strategies to inactivate E. coli O157:H7 on diverse surfaces in food processing and handling environments.",
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