Inhibition of Escherichia coli O157:H7 attachment by interactions between lactic acid bacteria and intestinal epithelial cells

Younghoon Kim, Sae Hun Kim, Kwang Youn Whang, Young Jun Kim, Sejong Oh

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

The intestinal epithelial cell (IEC) layer of the intestinal tract makes direct contact with a number of microbiota communities, including bacteria known to have deleterious health effects. IECs possess innate protective strategies against pathogenic challenge, which primarily involve the formation of a physicochemical barrier. Intestinal tract mucins are principal components of the mucus layer on epithelial surfaces, and perform a protective function against microbial damage. However, little is currently known regarding the interactions between probiotics/pathogens and epithelial cell mucins. The principal objective of this study was to determine the effects of Lactobacillus on the upregulation of MUC2 mucin and the subsequent inhibition of E. coli O157:H7 attachment to epithelial cells. In the current study, the attachment of E. coli O157:H7 to HT-29 intestinal epithelial cells was inhibited significantly by L. acidophilus A4 and its cell extracts. It is also important to note that the expression of MUC2 mucin was increased as the result of the addition of L. acidophilus A4 cell extracts (10.0 mg/ml), which also induced a significant reduction in the degree to which E. coli O157:H7 attached to epithelial cells. In addition, the mRNA levels of IL-8, IL-1β, and TNF-α in HT-29 cells were significantly induced by treatment with L. acidophilus A4 extracts. These results indicate that MUC2 mucin and cytokines are important regulatory factors in the immune systems of the gut, and that selected lactobacilli may be able to induce the upregulation of MUC2 mucin and specific cytokines, thereby inhibiting the attachment of E. coli O157:H7.

Original languageEnglish
Pages (from-to)1278-1285
Number of pages8
JournalJournal of microbiology and biotechnology
Volume18
Issue number7
Publication statusPublished - 2008 Jul 28

Keywords

  • Attachment
  • Cytokine
  • E. coli O157:H7
  • Lactobacillus acidophilus
  • MUC2
  • Reverse-transcription polymerase chain reaction

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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