Inhibition of Helicobacter pylori adhesion to human gastric adenocarcinoma epithelial cells by acidic polysaccharides from Artemisia capillaris and Panax ginseng

Ji Hye Lee, Kyung Park Eun, Chang Sub Uhm, Mi Sook Chung, Hyun Kim Kyung

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Helicobacter pylori specifically adheres to host cells, mainly based on carbohydrate-mediated cell-cell interactions. Previously, we investigated the anti-adhesive effect of polysaccharide fractions from Artemisia capillaris and Panax ginseng, using hemagglutination and enzyme-linked glycosorbent assays. In the present study, each active polysaccharide fraction was further purified, resulting in a single peak (fraction F2) using gel filtration FPLC, in which no protein content was detectable. Using scanning electron microscopy, we examined the inhibitory effects of these polysaccharides on the attachment of H. pylori to the human gastric adenocarcinoma epithelial cell line. The bacterial attachment to the cell line was inhibited by these polysaccharides in the range of the concentrations studied (0.2-2.8 mg/ mL), showing their minimum inhibitory concentration at as low as 0.2 mg/mL. The bacterial binding was inhibited more effectively by P. ginseng polysaccharides, than by those from A. capillaris. The purified polysaccharides contain similar sugar compositions and have high amounts of uronic acids. Our results suggest that acidic carbohydrates may play an important role in the inhibitory activity on H. pylori adhesion to host cells and that our established purification protocol can be applied to obtain active acidic polysaccharides from plant sources.

Original languageEnglish
Pages (from-to)615-619
Number of pages5
JournalPlanta Medica
Volume70
Issue number7
DOIs
Publication statusPublished - 2004 Jul 1

Fingerprint

Artemisia
Panax
Helicobacter pylori
Polysaccharides
Stomach
Adenocarcinoma
Adhesion
Epithelial Cells
Carbohydrates
Uronic Acids
Cell Line
Hemagglutination
Microbial Sensitivity Tests
Cell Communication
Sugars
Adhesives
Electron Scanning Microscopy
Purification
Gel Chromatography
Assays

Keywords

  • Acidic polysaccharide
  • Adhesion
  • Araliaceae
  • Artemisia capillaris
  • Asteracae
  • Helicobacter pylori
  • Human gastric adenocarcinoma epithelial cells
  • Panax ginseng

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this

Inhibition of Helicobacter pylori adhesion to human gastric adenocarcinoma epithelial cells by acidic polysaccharides from Artemisia capillaris and Panax ginseng. / Lee, Ji Hye; Eun, Kyung Park; Uhm, Chang Sub; Chung, Mi Sook; Kyung, Hyun Kim.

In: Planta Medica, Vol. 70, No. 7, 01.07.2004, p. 615-619.

Research output: Contribution to journalArticle

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