TY - JOUR
T1 - Inhibition of histone deacetylation alters Arabidopsis root growth in response to auxin via PIN1 degradation
AU - Nguyen, Hoai Nguyen
AU - Kim, Jun Hyeok
AU - Jeong, Chan Young
AU - Hong, Suk Whan
AU - Lee, Hojoung
N1 - Funding Information:
Acknowledgments We would like to thank to Dr. Kequiang Wu (National Taiwan University) for donating hda19 seeds. This work was supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (to Hojoung Lee, 2012; Grant #2012-112068-3) and by a grant from the National Research Foundation (to Suk-Whan Hong; Grant #2012R1A1A4A01006448).
PY - 2013/10
Y1 - 2013/10
N2 - Key message: Our results showed the histone deacetylase inhibitors (HDIs) control root development in Arabidopsis via regulation of PIN1 degradation. Epigenetic regulation plays a crucial role in the expression of many genes in response to exogenous or endogenous signals in plants as well as other organisms. One of epigenetic mechanisms is modifications of histone, such as acetylation and deacetylation, are catalyzed by histone acetyltransferase (HAT) and histone deacetylase (HDAC), respectively. The Arabidopsis HDACs, HDA6, and HDA19, were reported to function in physiological processes, including embryo development, abiotic stress response, and flowering. In this study, we demonstrated that histone deacetylase inhibitors (HDIs) inhibit primary root elongation and lateral root emergence. In response to HDIs treatment, the PIN1 protein was almost abolished in the root tip. However, the PIN1 gene did not show decreased expression in the presence of HDIs, whereas IAA genes exhibited increases in transcript levels. In contrast, we observed a stable level of gene expression of stress markers (KIN1 and COR15A) and a cell division marker (CYCB1). Taken together, these results suggest that epigenetic regulation may control auxin-mediated root development through the 26S proteasome-mediated degradation of PIN1 protein.
AB - Key message: Our results showed the histone deacetylase inhibitors (HDIs) control root development in Arabidopsis via regulation of PIN1 degradation. Epigenetic regulation plays a crucial role in the expression of many genes in response to exogenous or endogenous signals in plants as well as other organisms. One of epigenetic mechanisms is modifications of histone, such as acetylation and deacetylation, are catalyzed by histone acetyltransferase (HAT) and histone deacetylase (HDAC), respectively. The Arabidopsis HDACs, HDA6, and HDA19, were reported to function in physiological processes, including embryo development, abiotic stress response, and flowering. In this study, we demonstrated that histone deacetylase inhibitors (HDIs) inhibit primary root elongation and lateral root emergence. In response to HDIs treatment, the PIN1 protein was almost abolished in the root tip. However, the PIN1 gene did not show decreased expression in the presence of HDIs, whereas IAA genes exhibited increases in transcript levels. In contrast, we observed a stable level of gene expression of stress markers (KIN1 and COR15A) and a cell division marker (CYCB1). Taken together, these results suggest that epigenetic regulation may control auxin-mediated root development through the 26S proteasome-mediated degradation of PIN1 protein.
KW - Auxin
KW - Epigenetic
KW - PIN1
KW - Root development
KW - Sodium butyrate (NaB)
KW - Trichostatin A (TSA)
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U2 - 10.1007/s00299-013-1474-6
DO - 10.1007/s00299-013-1474-6
M3 - Article
C2 - 23820978
AN - SCOPUS:84884205486
VL - 32
SP - 1625
EP - 1636
JO - Plant Cell Reports
JF - Plant Cell Reports
SN - 0721-7714
IS - 10
ER -