Inhibition of interleukin-12 production in lipopolysaccharide-activated macrophages by curcumin

Bok Yun Kang, Su Wol Chung, Woon Jae Chung, Suhn Young Im, Seung Yong Hwang, Tae Sung Kim

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Pharmacological control of interleukin-12 production may be a key therapeutic strategy for modulating immunological diseases dominated by type-1 cytokine responses. In this study we investigated the effects of curcumin (1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6-heptadiene-3,5-dione) on the production of interleukin-12 from mouse macrophages stimulated with lipopolysaccharide. Curcumin potently inhibited the production of interleukin-12 in a dose-dependent manner. The effect of curcumin on interleukin-12 p40 promoter activation was analyzed by transfecting RAW264.7 monocytic cells with p40 promoter/reporter constructs. The repressive effect mapped to a region in the p40 promoter containing a binding site for nuclear factor κB (p40-κB). Furthermore, activation of macrophages by lipopolysaccharide resulted in markedly enhanced binding activity to the κB site, which significantly decreased upon addition of curcumin. These results suggest that curcumin-induced inhibition of interleukin-12 production in macrophages may explain some of the biological effects of curcumin including its anti-inflammatory activity. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)191-195
Number of pages5
JournalEuropean Journal of Pharmacology
Volume384
Issue number2-3
DOIs
Publication statusPublished - 1999 Nov 19
Externally publishedYes

Fingerprint

Curcumin
Interleukin-12
Lipopolysaccharides
Macrophages
Macrophage Activation
Immune System Diseases
Anti-Inflammatory Agents
Binding Sites
Pharmacology
Cytokines
adjuvant P40

Keywords

  • Curcumin
  • Interleukin-12
  • Macrophage
  • Nuclear factor κB

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Inhibition of interleukin-12 production in lipopolysaccharide-activated macrophages by curcumin. / Kang, Bok Yun; Chung, Su Wol; Chung, Woon Jae; Im, Suhn Young; Hwang, Seung Yong; Kim, Tae Sung.

In: European Journal of Pharmacology, Vol. 384, No. 2-3, 19.11.1999, p. 191-195.

Research output: Contribution to journalArticle

Kang, Bok Yun ; Chung, Su Wol ; Chung, Woon Jae ; Im, Suhn Young ; Hwang, Seung Yong ; Kim, Tae Sung. / Inhibition of interleukin-12 production in lipopolysaccharide-activated macrophages by curcumin. In: European Journal of Pharmacology. 1999 ; Vol. 384, No. 2-3. pp. 191-195.
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