Inhibition of interleukin-12 production in mouse macrophages via suppression of nuclear factor-κB binding activity by Phyllostachys nigra var. henonis

Seung Hyun Kim, Tae Sung Kim, Sung Jun Kim, Chi Nam Seong, Oh Hyung Lee, Hyo Jung Lee, Jin Cheol Yoo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing development and progression of disease in experimental models of autoimmunity. The acetone fraction prepared from bamboo, Phyllostachys nigra var. henonis, potently inhibited the Lipo polysaccharide (LPS)-induced IL-12 production from RAW264.7 monocytic cell-line in a dose-dependent manner. The repressive effect mapped to a region in the IL-12 gene promoter containing a binding site for NF-κB. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-κB site, which significantly decreased upon addition of the acetone fraction of Phyllostachys nigra var. henonis. This indicated that the acetone fraction inhibited IL-12 production in LPS-activated macrophages via inhibition of NF-κB binding activity.

Original languageEnglish
Pages (from-to)131-139
Number of pages9
JournalImmunopharmacology and Immunotoxicology
Volume29
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Macrophages
Interleukin-12
Acetone
Polysaccharides
Bamboo
Macrophage Activation
Autoimmunity
Disease Progression
Theoretical Models
Genes
Chemical activation
Binding Sites
Pharmacology
Cell Line
Therapeutics

Keywords

  • Bamboo Leaf
  • Interleukin-12 Production
  • Macrophage
  • Nuclear Factor-κB
  • Phyllostachys Nigra var. Henonis

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Immunology
  • Pharmacology
  • Toxicology

Cite this

Inhibition of interleukin-12 production in mouse macrophages via suppression of nuclear factor-κB binding activity by Phyllostachys nigra var. henonis. / Kim, Seung Hyun; Kim, Tae Sung; Kim, Sung Jun; Seong, Chi Nam; Lee, Oh Hyung; Lee, Hyo Jung; Yoo, Jin Cheol.

In: Immunopharmacology and Immunotoxicology, Vol. 29, No. 1, 01.01.2007, p. 131-139.

Research output: Contribution to journalArticle

Kim, Seung Hyun ; Kim, Tae Sung ; Kim, Sung Jun ; Seong, Chi Nam ; Lee, Oh Hyung ; Lee, Hyo Jung ; Yoo, Jin Cheol. / Inhibition of interleukin-12 production in mouse macrophages via suppression of nuclear factor-κB binding activity by Phyllostachys nigra var. henonis. In: Immunopharmacology and Immunotoxicology. 2007 ; Vol. 29, No. 1. pp. 131-139.
@article{a1b191a2f4eb4cc8a42c497d13968fb6,
title = "Inhibition of interleukin-12 production in mouse macrophages via suppression of nuclear factor-κB binding activity by Phyllostachys nigra var. henonis",
abstract = "Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing development and progression of disease in experimental models of autoimmunity. The acetone fraction prepared from bamboo, Phyllostachys nigra var. henonis, potently inhibited the Lipo polysaccharide (LPS)-induced IL-12 production from RAW264.7 monocytic cell-line in a dose-dependent manner. The repressive effect mapped to a region in the IL-12 gene promoter containing a binding site for NF-κB. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-κB site, which significantly decreased upon addition of the acetone fraction of Phyllostachys nigra var. henonis. This indicated that the acetone fraction inhibited IL-12 production in LPS-activated macrophages via inhibition of NF-κB binding activity.",
keywords = "Bamboo Leaf, Interleukin-12 Production, Macrophage, Nuclear Factor-κB, Phyllostachys Nigra var. Henonis",
author = "Kim, {Seung Hyun} and Kim, {Tae Sung} and Kim, {Sung Jun} and Seong, {Chi Nam} and Lee, {Oh Hyung} and Lee, {Hyo Jung} and Yoo, {Jin Cheol}",
year = "2007",
month = "1",
day = "1",
doi = "10.1080/08923970701283476",
language = "English",
volume = "29",
pages = "131--139",
journal = "Immunopharmacology and Immunotoxicology",
issn = "0892-3973",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - Inhibition of interleukin-12 production in mouse macrophages via suppression of nuclear factor-κB binding activity by Phyllostachys nigra var. henonis

AU - Kim, Seung Hyun

AU - Kim, Tae Sung

AU - Kim, Sung Jun

AU - Seong, Chi Nam

AU - Lee, Oh Hyung

AU - Lee, Hyo Jung

AU - Yoo, Jin Cheol

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing development and progression of disease in experimental models of autoimmunity. The acetone fraction prepared from bamboo, Phyllostachys nigra var. henonis, potently inhibited the Lipo polysaccharide (LPS)-induced IL-12 production from RAW264.7 monocytic cell-line in a dose-dependent manner. The repressive effect mapped to a region in the IL-12 gene promoter containing a binding site for NF-κB. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-κB site, which significantly decreased upon addition of the acetone fraction of Phyllostachys nigra var. henonis. This indicated that the acetone fraction inhibited IL-12 production in LPS-activated macrophages via inhibition of NF-κB binding activity.

AB - Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing development and progression of disease in experimental models of autoimmunity. The acetone fraction prepared from bamboo, Phyllostachys nigra var. henonis, potently inhibited the Lipo polysaccharide (LPS)-induced IL-12 production from RAW264.7 monocytic cell-line in a dose-dependent manner. The repressive effect mapped to a region in the IL-12 gene promoter containing a binding site for NF-κB. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-κB site, which significantly decreased upon addition of the acetone fraction of Phyllostachys nigra var. henonis. This indicated that the acetone fraction inhibited IL-12 production in LPS-activated macrophages via inhibition of NF-κB binding activity.

KW - Bamboo Leaf

KW - Interleukin-12 Production

KW - Macrophage

KW - Nuclear Factor-κB

KW - Phyllostachys Nigra var. Henonis

UR - http://www.scopus.com/inward/record.url?scp=34247642179&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247642179&partnerID=8YFLogxK

U2 - 10.1080/08923970701283476

DO - 10.1080/08923970701283476

M3 - Article

C2 - 17464773

AN - SCOPUS:34247642179

VL - 29

SP - 131

EP - 139

JO - Immunopharmacology and Immunotoxicology

JF - Immunopharmacology and Immunotoxicology

SN - 0892-3973

IS - 1

ER -