Inhibition of LPS-induced nitric oxide production by transduced Tat-arginine deiminase fusion protein in Raw 264.7 cells

Min Jung Lee, Dae Won Kim, Yeom Pyo Lee, Hoon Jae Jeong, Hye Won Kang, Min Jae Shin, Eun Jeong Sohn, Mi Jin Kim, Sang Ho Jang, Tae Cheon Kang, Moo Ho Won, Bon Hong Min, Sung Woo Cho, Kil Soo Lee, Jinseu Park, Won Sik Eum, Soo Young Choi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Arginine deiminase (ADI), an arginine-degrading enzyme, has anti-proliferative and anti-tumor activities and is capable of inhibiting the production of nitric oxide (NO). Modulation of nitric oxide (NO) production is considered a promising approach for the treatment of various diseases including cancer, inflammation and neuronal disorders. In this study, an ADI gene was fused with an HIV-1 Tat peptide in a bacterial expression vector to produce an genetic in-frame Tat-ADI fusion protein. When added exogenously to the culture media, the expressed and purified Tat-ADI fusion proteins were efficiently transduced into macrophage Raw 264.7 cells in a time- and dose-dependent manner. Furthermore, transduced Tat-ADI fusion proteins markedly increased cell viability in cells treated with lipopolysaccharide (LPS). This increase in viability was mediated by an inhibition of NO production. These results suggest that this Tat-ADI fusion protein can be used in protein therapies of NO-related disorders such as cancer, inflammation and neuronal diseases.

Original languageEnglish
Pages (from-to)286-292
Number of pages7
JournalBMB reports
Volume42
Issue number5
DOIs
Publication statusPublished - 2009 May

Keywords

  • Arginine deiminase (ADI)
  • HIV-1 Tat peptide
  • Inflammation
  • Nitric oxide synthase
  • Protein transduction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Lee, M. J., Kim, D. W., Lee, Y. P., Jeong, H. J., Kang, H. W., Shin, M. J., Sohn, E. J., Kim, M. J., Jang, S. H., Kang, T. C., Won, M. H., Min, B. H., Cho, S. W., Lee, K. S., Park, J., Eum, W. S., & Choi, S. Y. (2009). Inhibition of LPS-induced nitric oxide production by transduced Tat-arginine deiminase fusion protein in Raw 264.7 cells. BMB reports, 42(5), 286-292. https://doi.org/10.5483/BMBRep.2009.42.5.286