Inhibition of mouse macrophages interleukin-12 production: Suppression of nuclear factor-κB binding activity by a specific factor isolated from Scapharca broughtonii

Hyun Kim Seung, Tae Sung Kim, Woo Lee June, Wan H. Jung, Soo Park Nae, Jung Lee Hyo, Cheol Yoo Jin

Research output: Contribution to journalArticle

Abstract

Pharmacological inhibition of interleukin-12 (IL-12) production may allow a therapeutic strategy for preventing development and progression of disease in experimental models of autoimmunity. In this study we investigated the effects of an ethanol fraction of the Scapharca broughtonii, on the production of IL-12 by mouse macrophages stimulated with lipopolysaccharides (LPS). The ethanol fraction (S3) prepared from Scapharca broughtonii potently inhibited LPS-induced IL-12 production in the RAW264.7 monocyte cell-line in a dose-dependent manner. The activation effect of the ethanol fraction (S3) on the IL-12 gene promoter was analyzed by transfecting RAW264.7 cells with IL-12 gene promoter/luciferase constructs. The repressive effect mapped to a region in the IL-12 gene promoter that contained a binding site for NF-κB. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-κB site, which significantly decreased upon addition of the ethanol fraction, indicating that the ethanol fraction of the blood shell inhibited IL-12 production in LPS-activated macrophages via inhibition of NF-κB binding activity.

Original languageEnglish
Pages (from-to)350-354
Number of pages5
JournalArchives of Pharmacal Research
Volume30
Issue number3
Publication statusPublished - 2007 Mar 31

Fingerprint

Scapharca
Macrophages
Interleukin-12
Ethanol
Lipopolysaccharides
Genes
Chemical activation
Macrophage Activation
Luciferases
Autoimmunity
Disease Progression
Monocytes
Blood
Theoretical Models
Binding Sites
Pharmacology
Cell Line

Keywords

  • Blood shell
  • Interleukin-12
  • Macrophage
  • NF-κB
  • RAW264.7 cell
  • Scapharca broughtonii

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

Inhibition of mouse macrophages interleukin-12 production : Suppression of nuclear factor-κB binding activity by a specific factor isolated from Scapharca broughtonii. / Seung, Hyun Kim; Kim, Tae Sung; June, Woo Lee; Jung, Wan H.; Nae, Soo Park; Hyo, Jung Lee; Jin, Cheol Yoo.

In: Archives of Pharmacal Research, Vol. 30, No. 3, 31.03.2007, p. 350-354.

Research output: Contribution to journalArticle

Seung, Hyun Kim ; Kim, Tae Sung ; June, Woo Lee ; Jung, Wan H. ; Nae, Soo Park ; Hyo, Jung Lee ; Jin, Cheol Yoo. / Inhibition of mouse macrophages interleukin-12 production : Suppression of nuclear factor-κB binding activity by a specific factor isolated from Scapharca broughtonii. In: Archives of Pharmacal Research. 2007 ; Vol. 30, No. 3. pp. 350-354.
@article{a8c89756582f40b8a9c3441465b117b4,
title = "Inhibition of mouse macrophages interleukin-12 production: Suppression of nuclear factor-κB binding activity by a specific factor isolated from Scapharca broughtonii",
abstract = "Pharmacological inhibition of interleukin-12 (IL-12) production may allow a therapeutic strategy for preventing development and progression of disease in experimental models of autoimmunity. In this study we investigated the effects of an ethanol fraction of the Scapharca broughtonii, on the production of IL-12 by mouse macrophages stimulated with lipopolysaccharides (LPS). The ethanol fraction (S3) prepared from Scapharca broughtonii potently inhibited LPS-induced IL-12 production in the RAW264.7 monocyte cell-line in a dose-dependent manner. The activation effect of the ethanol fraction (S3) on the IL-12 gene promoter was analyzed by transfecting RAW264.7 cells with IL-12 gene promoter/luciferase constructs. The repressive effect mapped to a region in the IL-12 gene promoter that contained a binding site for NF-κB. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-κB site, which significantly decreased upon addition of the ethanol fraction, indicating that the ethanol fraction of the blood shell inhibited IL-12 production in LPS-activated macrophages via inhibition of NF-κB binding activity.",
keywords = "Blood shell, Interleukin-12, Macrophage, NF-κB, RAW264.7 cell, Scapharca broughtonii",
author = "Seung, {Hyun Kim} and Kim, {Tae Sung} and June, {Woo Lee} and Jung, {Wan H.} and Nae, {Soo Park} and Hyo, {Jung Lee} and Jin, {Cheol Yoo}",
year = "2007",
month = "3",
day = "31",
language = "English",
volume = "30",
pages = "350--354",
journal = "Archives of Pharmacal Research",
issn = "0253-6269",
publisher = "Pharmaceutical Society of Korea",
number = "3",

}

TY - JOUR

T1 - Inhibition of mouse macrophages interleukin-12 production

T2 - Suppression of nuclear factor-κB binding activity by a specific factor isolated from Scapharca broughtonii

AU - Seung, Hyun Kim

AU - Kim, Tae Sung

AU - June, Woo Lee

AU - Jung, Wan H.

AU - Nae, Soo Park

AU - Hyo, Jung Lee

AU - Jin, Cheol Yoo

PY - 2007/3/31

Y1 - 2007/3/31

N2 - Pharmacological inhibition of interleukin-12 (IL-12) production may allow a therapeutic strategy for preventing development and progression of disease in experimental models of autoimmunity. In this study we investigated the effects of an ethanol fraction of the Scapharca broughtonii, on the production of IL-12 by mouse macrophages stimulated with lipopolysaccharides (LPS). The ethanol fraction (S3) prepared from Scapharca broughtonii potently inhibited LPS-induced IL-12 production in the RAW264.7 monocyte cell-line in a dose-dependent manner. The activation effect of the ethanol fraction (S3) on the IL-12 gene promoter was analyzed by transfecting RAW264.7 cells with IL-12 gene promoter/luciferase constructs. The repressive effect mapped to a region in the IL-12 gene promoter that contained a binding site for NF-κB. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-κB site, which significantly decreased upon addition of the ethanol fraction, indicating that the ethanol fraction of the blood shell inhibited IL-12 production in LPS-activated macrophages via inhibition of NF-κB binding activity.

AB - Pharmacological inhibition of interleukin-12 (IL-12) production may allow a therapeutic strategy for preventing development and progression of disease in experimental models of autoimmunity. In this study we investigated the effects of an ethanol fraction of the Scapharca broughtonii, on the production of IL-12 by mouse macrophages stimulated with lipopolysaccharides (LPS). The ethanol fraction (S3) prepared from Scapharca broughtonii potently inhibited LPS-induced IL-12 production in the RAW264.7 monocyte cell-line in a dose-dependent manner. The activation effect of the ethanol fraction (S3) on the IL-12 gene promoter was analyzed by transfecting RAW264.7 cells with IL-12 gene promoter/luciferase constructs. The repressive effect mapped to a region in the IL-12 gene promoter that contained a binding site for NF-κB. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-κB site, which significantly decreased upon addition of the ethanol fraction, indicating that the ethanol fraction of the blood shell inhibited IL-12 production in LPS-activated macrophages via inhibition of NF-κB binding activity.

KW - Blood shell

KW - Interleukin-12

KW - Macrophage

KW - NF-κB

KW - RAW264.7 cell

KW - Scapharca broughtonii

UR - http://www.scopus.com/inward/record.url?scp=34147107080&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34147107080&partnerID=8YFLogxK

M3 - Article

C2 - 17424942

AN - SCOPUS:34147107080

VL - 30

SP - 350

EP - 354

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

SN - 0253-6269

IS - 3

ER -