Inhibition of protein tyrosine phosphatase 1B by ursane-type triterpenes isolated from Symplocos paniculata

MinKyun Na, Seungmi Yang, Long He, Hyuncheol Oh, Beom Seok Kim, Won Keun Oh, Bo Yeon Kim, Jong Seog Ahn

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Inhibition of protein tyrosine phosphatase IB (PTP1B) has been proposed as a therapy for treatment of type 2 diabetes and obesity. Bioassay-guided fractionation of the MeOH extract of the leaves and stems of Symplocos paniculata (Thunb.) Miq. (Symplocaceae), using an in vitro PTP1B inhibitory assay, resulted in the isolation of three ursane-type triterpenes, ursolic acid (1), corosolic acid (2) and 2α,3α,19α,23-tetrahydroxyurs-12- en-28-oic acid (3). Compounds 1-3 inhibited PTP1B with IC50 values of 3.8 ± 0.5, 7.2 ± 0.8 and 42.1 ± 1.5 μM, respectively. Kinetic studies suggest that 1 is a competitive inhibitor with a Ki value of 2.0 μM, whereas 2 is a mixed-type inhibitor of PTP1B. Our results indicate that the substitution of hydroxy groups on the ursane-type triterpenes is responsible for the loss of activity, and thus 1 and 2 possessing only one or two hydroxy groups can be potential PTP1B inhibitors.

Original languageEnglish
Pages (from-to)261-263
Number of pages3
JournalPlanta Medica
Volume72
Issue number3
DOIs
Publication statusPublished - 2006 Feb 1
Externally publishedYes

Fingerprint

Non-Receptor Type 1 Protein Tyrosine Phosphatase
protein-tyrosine-phosphatase
Triterpenes
triterpenoids
Symplocaceae
ursolic acid
Protein Tyrosine Phosphatases
Bioassay
acids
Fractionation
Medical problems
noninsulin-dependent diabetes mellitus
leaf extracts
Biological Assay
Type 2 Diabetes Mellitus
Inhibitory Concentration 50
inhibitory concentration 50
Assays
fractionation
obesity

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Inhibition of protein tyrosine phosphatase 1B by ursane-type triterpenes isolated from Symplocos paniculata. / Na, MinKyun; Yang, Seungmi; He, Long; Oh, Hyuncheol; Kim, Beom Seok; Oh, Won Keun; Kim, Bo Yeon; Ahn, Jong Seog.

In: Planta Medica, Vol. 72, No. 3, 01.02.2006, p. 261-263.

Research output: Contribution to journalArticle

Na, MinKyun ; Yang, Seungmi ; He, Long ; Oh, Hyuncheol ; Kim, Beom Seok ; Oh, Won Keun ; Kim, Bo Yeon ; Ahn, Jong Seog. / Inhibition of protein tyrosine phosphatase 1B by ursane-type triterpenes isolated from Symplocos paniculata. In: Planta Medica. 2006 ; Vol. 72, No. 3. pp. 261-263.
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