Inhibition of receptor internalization attenuates the TNFα-induced ROS generation in non-phagocytic cells

Chang Hoon Woo, Tae Hee Kim, Jung A. Choi, Ho Cheol Ryu, Jung Eun Lee, Hye Jin You, Yun Soo Bae, Jae-Hong Kim

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) are important regulatory molecules implicated in the signaling cascade triggered by tumor necrosis factor (TNF)α, although the events through which TNFα induces ROS generation are not well characterized. Here, we report that TNFα-induced ROS production was blocked by pretreatment with internalization inhibitor monodansyl cadaverine (MDC). Similarly, a transient expression of a GTP-binding and hydrolysis-defective dynamin mutant (dynaminK44A) that had been shown to be defective in internalization significantly attenuated the TNFα-induced intracellular ROS production. Importantly, the inhibition of receptor internalization suppressed TNFα signaling to mitogen-activated protein kinases (MAPKs) stimulation. Together, our results suggest that receptor internalization is somehow necessary for the TNFα-induced ROS generation and subsequent intracellular downstream signaling in non-phagocytes.

Original languageEnglish
Pages (from-to)972-978
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume351
Issue number4
DOIs
Publication statusPublished - 2006 Dec 29

Fingerprint

Reactive Oxygen Species
Tumor Necrosis Factor-alpha
Cadaverine
Dynamins
Guanosine Triphosphate
Mitogen-Activated Protein Kinases
Hydrolysis
Molecules

Keywords

  • Non-phagocytes
  • Nox
  • Receptor internalization
  • ROS
  • Signal transduction
  • TNFα

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Inhibition of receptor internalization attenuates the TNFα-induced ROS generation in non-phagocytic cells. / Woo, Chang Hoon; Kim, Tae Hee; Choi, Jung A.; Ryu, Ho Cheol; Lee, Jung Eun; You, Hye Jin; Bae, Yun Soo; Kim, Jae-Hong.

In: Biochemical and Biophysical Research Communications, Vol. 351, No. 4, 29.12.2006, p. 972-978.

Research output: Contribution to journalArticle

Woo, Chang Hoon ; Kim, Tae Hee ; Choi, Jung A. ; Ryu, Ho Cheol ; Lee, Jung Eun ; You, Hye Jin ; Bae, Yun Soo ; Kim, Jae-Hong. / Inhibition of receptor internalization attenuates the TNFα-induced ROS generation in non-phagocytic cells. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 351, No. 4. pp. 972-978.
@article{5ab8483237604c2e8627af17ddf832d4,
title = "Inhibition of receptor internalization attenuates the TNFα-induced ROS generation in non-phagocytic cells",
abstract = "Reactive oxygen species (ROS) are important regulatory molecules implicated in the signaling cascade triggered by tumor necrosis factor (TNF)α, although the events through which TNFα induces ROS generation are not well characterized. Here, we report that TNFα-induced ROS production was blocked by pretreatment with internalization inhibitor monodansyl cadaverine (MDC). Similarly, a transient expression of a GTP-binding and hydrolysis-defective dynamin mutant (dynaminK44A) that had been shown to be defective in internalization significantly attenuated the TNFα-induced intracellular ROS production. Importantly, the inhibition of receptor internalization suppressed TNFα signaling to mitogen-activated protein kinases (MAPKs) stimulation. Together, our results suggest that receptor internalization is somehow necessary for the TNFα-induced ROS generation and subsequent intracellular downstream signaling in non-phagocytes.",
keywords = "Non-phagocytes, Nox, Receptor internalization, ROS, Signal transduction, TNFα",
author = "Woo, {Chang Hoon} and Kim, {Tae Hee} and Choi, {Jung A.} and Ryu, {Ho Cheol} and Lee, {Jung Eun} and You, {Hye Jin} and Bae, {Yun Soo} and Jae-Hong Kim",
year = "2006",
month = "12",
day = "29",
doi = "10.1016/j.bbrc.2006.10.154",
language = "English",
volume = "351",
pages = "972--978",
journal = "The BMJ",
issn = "0730-6512",
publisher = "Kluwer Academic Publishers",
number = "4",

}

TY - JOUR

T1 - Inhibition of receptor internalization attenuates the TNFα-induced ROS generation in non-phagocytic cells

AU - Woo, Chang Hoon

AU - Kim, Tae Hee

AU - Choi, Jung A.

AU - Ryu, Ho Cheol

AU - Lee, Jung Eun

AU - You, Hye Jin

AU - Bae, Yun Soo

AU - Kim, Jae-Hong

PY - 2006/12/29

Y1 - 2006/12/29

N2 - Reactive oxygen species (ROS) are important regulatory molecules implicated in the signaling cascade triggered by tumor necrosis factor (TNF)α, although the events through which TNFα induces ROS generation are not well characterized. Here, we report that TNFα-induced ROS production was blocked by pretreatment with internalization inhibitor monodansyl cadaverine (MDC). Similarly, a transient expression of a GTP-binding and hydrolysis-defective dynamin mutant (dynaminK44A) that had been shown to be defective in internalization significantly attenuated the TNFα-induced intracellular ROS production. Importantly, the inhibition of receptor internalization suppressed TNFα signaling to mitogen-activated protein kinases (MAPKs) stimulation. Together, our results suggest that receptor internalization is somehow necessary for the TNFα-induced ROS generation and subsequent intracellular downstream signaling in non-phagocytes.

AB - Reactive oxygen species (ROS) are important regulatory molecules implicated in the signaling cascade triggered by tumor necrosis factor (TNF)α, although the events through which TNFα induces ROS generation are not well characterized. Here, we report that TNFα-induced ROS production was blocked by pretreatment with internalization inhibitor monodansyl cadaverine (MDC). Similarly, a transient expression of a GTP-binding and hydrolysis-defective dynamin mutant (dynaminK44A) that had been shown to be defective in internalization significantly attenuated the TNFα-induced intracellular ROS production. Importantly, the inhibition of receptor internalization suppressed TNFα signaling to mitogen-activated protein kinases (MAPKs) stimulation. Together, our results suggest that receptor internalization is somehow necessary for the TNFα-induced ROS generation and subsequent intracellular downstream signaling in non-phagocytes.

KW - Non-phagocytes

KW - Nox

KW - Receptor internalization

KW - ROS

KW - Signal transduction

KW - TNFα

UR - http://www.scopus.com/inward/record.url?scp=33750820203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750820203&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.10.154

DO - 10.1016/j.bbrc.2006.10.154

M3 - Article

VL - 351

SP - 972

EP - 978

JO - The BMJ

JF - The BMJ

SN - 0730-6512

IS - 4

ER -