Inhibition of sensory neuronal TRPs contributes to anti-nociception by butamben

Sangsu Bang, Tae Jin Yang, Sungjae Yoo, Tae Hwe Heo, Sun Wook Hwang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Butamben (n-butyl-p-aminobenzoic acid) is a pain-relieving local anesthetic for topical use. Blockade of voltage-gated channel expressed in the peripheral sensory neurons has been suggested as a mechanism of action. Its effects on another sensory neuronal channel family, transient receptor potential (TRP) have remained unclear. In this study we attempted to address this question using six sensory neuronal TRP channel-expressing heterologous systems, cultured sensory neurons and TRP-mediated acute animal pain tests. In Ca 2+ imaging and whole cell electrophysiology, TRPA1 and TRPV4 were blocked by micromolar butamben. Butamben also activated TRPA1 at millimolar concentrations. The inhibitory effects on the two TRP channels were reproducible in sensory neurons. Moreover, butamben attenuated acute animal pain behaviors in a TRPA1- or TRPV4-dependent manner. Para-aminobenzoic acid (PABA), an analog of a simpler chemical structure, displayed similar in vitro and in vivo properties, suggestive that chemical structure is important for the two TRP-specificity. Our findings suggest that inhibition of TRPA1 and TRPV4 contribute to the peripheral analgesic mechanisms of butamben.

Original languageEnglish
Pages (from-to)297-302
Number of pages6
JournalNeuroscience Letters
Volume506
Issue number2
DOIs
Publication statusPublished - 2012 Jan 11

Fingerprint

4-Aminobenzoic Acid
Transient Receptor Potential Channels
Nociception
Sensory Receptor Cells
Acute Pain
Animal Behavior
Electrophysiology
Local Anesthetics
Analgesics
Pain
Inhibition (Psychology)
butamben

Keywords

  • Butamben
  • Pain
  • Sensory neuron
  • TRPA1
  • TRPV4

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inhibition of sensory neuronal TRPs contributes to anti-nociception by butamben. / Bang, Sangsu; Yang, Tae Jin; Yoo, Sungjae; Heo, Tae Hwe; Hwang, Sun Wook.

In: Neuroscience Letters, Vol. 506, No. 2, 11.01.2012, p. 297-302.

Research output: Contribution to journalArticle

Bang, Sangsu ; Yang, Tae Jin ; Yoo, Sungjae ; Heo, Tae Hwe ; Hwang, Sun Wook. / Inhibition of sensory neuronal TRPs contributes to anti-nociception by butamben. In: Neuroscience Letters. 2012 ; Vol. 506, No. 2. pp. 297-302.
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