Inhibition of the type III secretion system of Pseudomonas syringae pv. tomato DC3000 by resveratrol oligomers identified in Vitis vinifera L

Ji Eun Kang, Byeong Jun Jeon, Min Young Park, Hye Ji Yang, Jaeyoung Kwon, Dong Ho Lee, Beom Seok Kim

Research output: Contribution to journalArticle

Abstract

BACKGROUND: The bacterial type III secretion system (T3SS) is one of the virulence determinants of Gram-negative bacteria through which various effector and virulence proteins are translocated into host cells. RESULTS: We constructed an assay system to screen inhibitors of hrpA gene expression (a structural gene of Hrp pili) in Pseudomonas syringae pv. tomato DC3000. In a plant extract library screening, the root extract of Vitis vinifera L. displayed the most prominent activity. Three resveratrol oligomers, hopeaphenol, isohopeaphenol and ampelopsin A, were identified in grapevine root extract, which significantly reduced the transcription levels of the hrpA, hrpL and hopP1 genes without growth retardation. Additional resveratrol derivatives identified in other plant extracts were also examined for their inhibitory effect on hrpA expression. Another resveratrol oligomer, kobophenol A, also inhibited the transcription of the hrpA gene and other T3SS-related genes, while resveratrol monomers (resveratrol and piceatannol) were not effective. The severity of bacterial specks was reduced by each hopeaphenol, isohopeaphenol and ampelopsin A treatment. CONCLUSION: These results show the potential of resveratrol derivatives as anti-virulence agents for the control of plant diseases.

Original languageEnglish
Pages (from-to)2294-2303
Number of pages10
JournalPest Management Science
Volume76
Issue number7
DOIs
Publication statusPublished - 2020 Jul 1

Keywords

  • Pseudomonas syringae pv. tomato
  • bacterial speck
  • resveratrol oligomer
  • type III secretion system inhibitor

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

Fingerprint Dive into the research topics of 'Inhibition of the type III secretion system of Pseudomonas syringae pv. tomato DC3000 by resveratrol oligomers identified in Vitis vinifera L'. Together they form a unique fingerprint.

  • Cite this