Inhibitor effects on molecular beacon-based mercury assays for tuning of detection range

Van Sang Le, Ji Eun Jeong, Boram Kim, Jiae Lee, Kwangseuk Kyhm, Han Young Woo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A series of inhibitors were incorporated to modulate the detection range of mercury assay. A single-strand DNA-based mercury probe (ssDNA-Hg) containing 5 thymine (T) bases at both termini, which forms a hairpin structure in the presence of mercuric (Hg 2+ ) ions via the T⋯Hg 2+ ⋯T interaction, was designed. Although many previous studies focused on mercury detection to improve the sensitivity and selectivity, few studies have been reported to tune the detection range to cover a wide range of target concentrations in different environments. In the present study, a new Hg 2+ detection scheme was designed to extend, narrow and shift the range of mercury detection using inhibitors. The complementary DNA (ssDNA-C) and cationic conjugated polyelectrolyte (CPE) induced a shift or broadening of the spectral response range by functioning as an allosteric inhibitor. Iodide and cyanide ions worked as efficient depletants, adjusting the binding constant of ssDNA-Hg and Hg 2+ (1.1 × 10 7  M −1 ) by ∼10 times (up to ∼1.4 × 10 6  M −1 ) in the presence of I or CN ions, resulting in a large shift in the detection range according to [I ] or [CN ] with a sharp titration curve via a sequestration mechanism. Similar Hg 2+ detection range shift was successfully demonstrated using same series of inhibitors in real samples such as Han River and tap water. This study suggests that the binding affinity of probe can be tuned toward a target and the resulting detection response range can be controlled using a range of inhibitors.

Original languageEnglish
Pages (from-to)810-817
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume240
DOIs
Publication statusPublished - 2017 Mar 1

Fingerprint

beacons
Mercury
inhibitors
Assays
Tuning
tuning
Ions
DNA
Cyanides
Polyelectrolytes
Titration
Thymine
shift
Iodides
Rivers
Complementary DNA
complementary DNA
ions
thymine
Water

Keywords

  • Conjugated polyelectrolytes
  • Detection range
  • Inhibitor
  • Mercury
  • Molecular beacon

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Inhibitor effects on molecular beacon-based mercury assays for tuning of detection range. / Le, Van Sang; Jeong, Ji Eun; Kim, Boram; Lee, Jiae; Kyhm, Kwangseuk; Woo, Han Young.

In: Sensors and Actuators, B: Chemical, Vol. 240, 01.03.2017, p. 810-817.

Research output: Contribution to journalArticle

Le, Van Sang ; Jeong, Ji Eun ; Kim, Boram ; Lee, Jiae ; Kyhm, Kwangseuk ; Woo, Han Young. / Inhibitor effects on molecular beacon-based mercury assays for tuning of detection range. In: Sensors and Actuators, B: Chemical. 2017 ; Vol. 240. pp. 810-817.
@article{b4e658cae3cb455b95cdad4c6164f1f4,
title = "Inhibitor effects on molecular beacon-based mercury assays for tuning of detection range",
abstract = "A series of inhibitors were incorporated to modulate the detection range of mercury assay. A single-strand DNA-based mercury probe (ssDNA-Hg) containing 5 thymine (T) bases at both termini, which forms a hairpin structure in the presence of mercuric (Hg 2+ ) ions via the T⋯Hg 2+ ⋯T interaction, was designed. Although many previous studies focused on mercury detection to improve the sensitivity and selectivity, few studies have been reported to tune the detection range to cover a wide range of target concentrations in different environments. In the present study, a new Hg 2+ detection scheme was designed to extend, narrow and shift the range of mercury detection using inhibitors. The complementary DNA (ssDNA-C) and cationic conjugated polyelectrolyte (CPE) induced a shift or broadening of the spectral response range by functioning as an allosteric inhibitor. Iodide and cyanide ions worked as efficient depletants, adjusting the binding constant of ssDNA-Hg and Hg 2+ (1.1 × 10 7  M −1 ) by ∼10 times (up to ∼1.4 × 10 6  M −1 ) in the presence of I − or CN − ions, resulting in a large shift in the detection range according to [I − ] or [CN − ] with a sharp titration curve via a sequestration mechanism. Similar Hg 2+ detection range shift was successfully demonstrated using same series of inhibitors in real samples such as Han River and tap water. This study suggests that the binding affinity of probe can be tuned toward a target and the resulting detection response range can be controlled using a range of inhibitors.",
keywords = "Conjugated polyelectrolytes, Detection range, Inhibitor, Mercury, Molecular beacon",
author = "Le, {Van Sang} and Jeong, {Ji Eun} and Boram Kim and Jiae Lee and Kwangseuk Kyhm and Woo, {Han Young}",
year = "2017",
month = "3",
day = "1",
doi = "10.1016/j.snb.2016.09.034",
language = "English",
volume = "240",
pages = "810--817",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",

}

TY - JOUR

T1 - Inhibitor effects on molecular beacon-based mercury assays for tuning of detection range

AU - Le, Van Sang

AU - Jeong, Ji Eun

AU - Kim, Boram

AU - Lee, Jiae

AU - Kyhm, Kwangseuk

AU - Woo, Han Young

PY - 2017/3/1

Y1 - 2017/3/1

N2 - A series of inhibitors were incorporated to modulate the detection range of mercury assay. A single-strand DNA-based mercury probe (ssDNA-Hg) containing 5 thymine (T) bases at both termini, which forms a hairpin structure in the presence of mercuric (Hg 2+ ) ions via the T⋯Hg 2+ ⋯T interaction, was designed. Although many previous studies focused on mercury detection to improve the sensitivity and selectivity, few studies have been reported to tune the detection range to cover a wide range of target concentrations in different environments. In the present study, a new Hg 2+ detection scheme was designed to extend, narrow and shift the range of mercury detection using inhibitors. The complementary DNA (ssDNA-C) and cationic conjugated polyelectrolyte (CPE) induced a shift or broadening of the spectral response range by functioning as an allosteric inhibitor. Iodide and cyanide ions worked as efficient depletants, adjusting the binding constant of ssDNA-Hg and Hg 2+ (1.1 × 10 7  M −1 ) by ∼10 times (up to ∼1.4 × 10 6  M −1 ) in the presence of I − or CN − ions, resulting in a large shift in the detection range according to [I − ] or [CN − ] with a sharp titration curve via a sequestration mechanism. Similar Hg 2+ detection range shift was successfully demonstrated using same series of inhibitors in real samples such as Han River and tap water. This study suggests that the binding affinity of probe can be tuned toward a target and the resulting detection response range can be controlled using a range of inhibitors.

AB - A series of inhibitors were incorporated to modulate the detection range of mercury assay. A single-strand DNA-based mercury probe (ssDNA-Hg) containing 5 thymine (T) bases at both termini, which forms a hairpin structure in the presence of mercuric (Hg 2+ ) ions via the T⋯Hg 2+ ⋯T interaction, was designed. Although many previous studies focused on mercury detection to improve the sensitivity and selectivity, few studies have been reported to tune the detection range to cover a wide range of target concentrations in different environments. In the present study, a new Hg 2+ detection scheme was designed to extend, narrow and shift the range of mercury detection using inhibitors. The complementary DNA (ssDNA-C) and cationic conjugated polyelectrolyte (CPE) induced a shift or broadening of the spectral response range by functioning as an allosteric inhibitor. Iodide and cyanide ions worked as efficient depletants, adjusting the binding constant of ssDNA-Hg and Hg 2+ (1.1 × 10 7  M −1 ) by ∼10 times (up to ∼1.4 × 10 6  M −1 ) in the presence of I − or CN − ions, resulting in a large shift in the detection range according to [I − ] or [CN − ] with a sharp titration curve via a sequestration mechanism. Similar Hg 2+ detection range shift was successfully demonstrated using same series of inhibitors in real samples such as Han River and tap water. This study suggests that the binding affinity of probe can be tuned toward a target and the resulting detection response range can be controlled using a range of inhibitors.

KW - Conjugated polyelectrolytes

KW - Detection range

KW - Inhibitor

KW - Mercury

KW - Molecular beacon

UR - http://www.scopus.com/inward/record.url?scp=84987942293&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84987942293&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2016.09.034

DO - 10.1016/j.snb.2016.09.034

M3 - Article

VL - 240

SP - 810

EP - 817

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

ER -