Abstract
Three guaianolide type sesquiterpene lactones, 3α,4α- epoxyrupicolins C-E (1-3), together with six known sesquiterpenes (4-9) were isolated and identified from the methanol extract of the aerial parts of Artemisia sylvatica, and their biological activities were reported. Three guaianolide sesquiterpene lactones, 3α,4α-epoxyrupicolins C-E, together with six known sesquiterpenes, artemisolide, 3-methoxytanapartholide, deacetyllaurenobiolide, moxartenolide as well as arteminolides B and D were isolated by bioassay-guided fractionation from the methanol extract of the aerial parts of Artemisia sylvatica using the NF-κB mediated reporter gene assay. All isolated compounds displayed inhibitory activity on the LPS-induced NF-κB activation, NO production, and TNF-α production with IC 50 values of 0.49-7.17, 1.46-6.16, and 3.19-27.76 μM, respectively, in RAW264.7 cells. It was also established that arteminolide B suppressed the expression of NF-κB target genes such as iNOS and COX-2. This is the first report of NF-κB inhibitory activities of these compounds and supports the pharmacological use of Artemisia sylvatica, which has been employed as an herbal medicine for the treatment of inflammation.
Original language | English |
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Pages (from-to) | 2247-2253 |
Number of pages | 7 |
Journal | Phytochemistry |
Volume | 65 |
Issue number | 15 |
DOIs | |
Publication status | Published - 2004 Aug |
Externally published | Yes |
Keywords
- Artemisia sylvatica
- COX-2
- Compositae
- NF-κB
- NO
- Sesquiterpene lactone
- TNF-α
- iNOS
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Plant Science
- Horticulture