Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages

Jin Woo Lee, Ji Hoon Lee, Chul Lee, Qinghao Jin, Dongho Lee, Youngsoo Kim, Jin Tae Hong, Mi Kyeong Lee, Bang Yeon Hwang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 4′,7-dihydroxyflavone (8), calycosin (9), and genistein (10). The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis. Compounds 1-4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 13.6 to 33.0 μM in RAW 264.7 macrophages.

Original languageEnglish
Pages (from-to)960-962
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume25
Issue number4
DOIs
Publication statusPublished - 2015 Feb 15

Fingerprint

Pterocarpans
Sophora
Spectroscopic analysis
Genistein
Macrophages
Fractionation
Glycosides
Bioactivity
Inhibitory Concentration 50
Hydrolysis
Nitric Oxide
Nuclear magnetic resonance
Acids
sophoranone
medioresinol
inermin
7,3'-dihydroxy-4'-methoxyisoflavone
syringaresinol
pinoresinol

Keywords

  • Leguminosae
  • Nitric oxide production inhibitor
  • Pterocarpan
  • Sophora tonkinensis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages. / Lee, Jin Woo; Lee, Ji Hoon; Lee, Chul; Jin, Qinghao; Lee, Dongho; Kim, Youngsoo; Hong, Jin Tae; Lee, Mi Kyeong; Hwang, Bang Yeon.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 25, No. 4, 15.02.2015, p. 960-962.

Research output: Contribution to journalArticle

Lee, Jin Woo ; Lee, Ji Hoon ; Lee, Chul ; Jin, Qinghao ; Lee, Dongho ; Kim, Youngsoo ; Hong, Jin Tae ; Lee, Mi Kyeong ; Hwang, Bang Yeon. / Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages. In: Bioorganic and Medicinal Chemistry Letters. 2015 ; Vol. 25, No. 4. pp. 960-962.
@article{5f4c916e43ac4cb38dec4acd9332b566,
title = "Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages",
abstract = "Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 4′,7-dihydroxyflavone (8), calycosin (9), and genistein (10). The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis. Compounds 1-4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 13.6 to 33.0 μM in RAW 264.7 macrophages.",
keywords = "Leguminosae, Nitric oxide production inhibitor, Pterocarpan, Sophora tonkinensis",
author = "Lee, {Jin Woo} and Lee, {Ji Hoon} and Chul Lee and Qinghao Jin and Dongho Lee and Youngsoo Kim and Hong, {Jin Tae} and Lee, {Mi Kyeong} and Hwang, {Bang Yeon}",
year = "2015",
month = "2",
day = "15",
doi = "10.1016/j.bmcl.2014.12.012",
language = "English",
volume = "25",
pages = "960--962",
journal = "Bioorganic and Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages

AU - Lee, Jin Woo

AU - Lee, Ji Hoon

AU - Lee, Chul

AU - Jin, Qinghao

AU - Lee, Dongho

AU - Kim, Youngsoo

AU - Hong, Jin Tae

AU - Lee, Mi Kyeong

AU - Hwang, Bang Yeon

PY - 2015/2/15

Y1 - 2015/2/15

N2 - Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 4′,7-dihydroxyflavone (8), calycosin (9), and genistein (10). The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis. Compounds 1-4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 13.6 to 33.0 μM in RAW 264.7 macrophages.

AB - Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 4′,7-dihydroxyflavone (8), calycosin (9), and genistein (10). The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis. Compounds 1-4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 13.6 to 33.0 μM in RAW 264.7 macrophages.

KW - Leguminosae

KW - Nitric oxide production inhibitor

KW - Pterocarpan

KW - Sophora tonkinensis

UR - http://www.scopus.com/inward/record.url?scp=84964284458&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964284458&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2014.12.012

DO - 10.1016/j.bmcl.2014.12.012

M3 - Article

C2 - 25592708

AN - SCOPUS:84964284458

VL - 25

SP - 960

EP - 962

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

SN - 0960-894X

IS - 4

ER -