Inhibitory effect of 2-arylbenzofurans from the Mori Cortex Radicis (moraceae) on oxygen glucose deprivation (OGD)-induced cell death of SH-SY5Y cells

Hak Ju Lee, Da Hyun Lyu, Uk Koo, Sung Jin Lee, Seong Su Hong, Kemok Kim, Kyeong Ho Kim, Dongho Lee, Woongchon Mar

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Three known 2-arylbenzofurans, moracin P (1), moracin O (2) and mulberrofuran Q (3) were isolated from the MeOH extract of the Mori Cortex Radicis. These compounds 1-3 enhanced cell viability in dose-dependent manner against oxygen-glucose deprivation (OGD)-induced cell death in neuroblastoma SH-SY5Y cells, which was measured by MTT reduction assay. (EC50 values of 10.4, 12.6, and 15.9 μM, respectively). In addition, the compounds 1-3 were examined for their inhibitory effect on OGD-induced ROS production by FACS analysis. We observed these compounds reduced ROS production in OGD-induced cell death (IC50 values of 1.9, 0.3 and 12.1 μM, respectively). Consequently, reactive oxygen species (ROS) were overexpressed in OGD-induced cells and all three compounds reduced ROS induced by OGD in dosedependent manner. Taken together, compounds 1-3 might protect neuronal cell death against the oxidative stress induced by OGD, though further studies in vitro and in vivo models are necessary.

Original languageEnglish
Pages (from-to)1373-1380
Number of pages8
JournalArchives of Pharmacal Research
Volume34
Issue number8
DOIs
Publication statusPublished - 2011 Aug 1

Fingerprint

Moraceae
Morus
Cell death
Cell Death
Oxygen
Glucose
Reactive Oxygen Species
Oxidative stress
Neuroblastoma
Inhibitory Concentration 50
Assays
Cell Survival
Oxidative Stress
Cells

Keywords

  • 2-Arylbenzofurans
  • Moraceae
  • Mori Cortex Radicis
  • MTT
  • Oxygen-glucose deprivation
  • ROS

ASJC Scopus subject areas

  • Drug Discovery
  • Molecular Medicine
  • Organic Chemistry

Cite this

Inhibitory effect of 2-arylbenzofurans from the Mori Cortex Radicis (moraceae) on oxygen glucose deprivation (OGD)-induced cell death of SH-SY5Y cells. / Lee, Hak Ju; Lyu, Da Hyun; Koo, Uk; Lee, Sung Jin; Hong, Seong Su; Kim, Kemok; Kim, Kyeong Ho; Lee, Dongho; Mar, Woongchon.

In: Archives of Pharmacal Research, Vol. 34, No. 8, 01.08.2011, p. 1373-1380.

Research output: Contribution to journalArticle

Lee, Hak Ju ; Lyu, Da Hyun ; Koo, Uk ; Lee, Sung Jin ; Hong, Seong Su ; Kim, Kemok ; Kim, Kyeong Ho ; Lee, Dongho ; Mar, Woongchon. / Inhibitory effect of 2-arylbenzofurans from the Mori Cortex Radicis (moraceae) on oxygen glucose deprivation (OGD)-induced cell death of SH-SY5Y cells. In: Archives of Pharmacal Research. 2011 ; Vol. 34, No. 8. pp. 1373-1380.
@article{22f54afa6ea2451c8b5b3f4df51c4e5d,
title = "Inhibitory effect of 2-arylbenzofurans from the Mori Cortex Radicis (moraceae) on oxygen glucose deprivation (OGD)-induced cell death of SH-SY5Y cells",
abstract = "Three known 2-arylbenzofurans, moracin P (1), moracin O (2) and mulberrofuran Q (3) were isolated from the MeOH extract of the Mori Cortex Radicis. These compounds 1-3 enhanced cell viability in dose-dependent manner against oxygen-glucose deprivation (OGD)-induced cell death in neuroblastoma SH-SY5Y cells, which was measured by MTT reduction assay. (EC50 values of 10.4, 12.6, and 15.9 μM, respectively). In addition, the compounds 1-3 were examined for their inhibitory effect on OGD-induced ROS production by FACS analysis. We observed these compounds reduced ROS production in OGD-induced cell death (IC50 values of 1.9, 0.3 and 12.1 μM, respectively). Consequently, reactive oxygen species (ROS) were overexpressed in OGD-induced cells and all three compounds reduced ROS induced by OGD in dosedependent manner. Taken together, compounds 1-3 might protect neuronal cell death against the oxidative stress induced by OGD, though further studies in vitro and in vivo models are necessary.",
keywords = "2-Arylbenzofurans, Moraceae, Mori Cortex Radicis, MTT, Oxygen-glucose deprivation, ROS",
author = "Lee, {Hak Ju} and Lyu, {Da Hyun} and Uk Koo and Lee, {Sung Jin} and Hong, {Seong Su} and Kemok Kim and Kim, {Kyeong Ho} and Dongho Lee and Woongchon Mar",
year = "2011",
month = "8",
day = "1",
doi = "10.1007/s12272-011-0818-4",
language = "English",
volume = "34",
pages = "1373--1380",
journal = "Archives of Pharmacal Research",
issn = "0253-6269",
publisher = "Pharmaceutical Society of Korea",
number = "8",

}

TY - JOUR

T1 - Inhibitory effect of 2-arylbenzofurans from the Mori Cortex Radicis (moraceae) on oxygen glucose deprivation (OGD)-induced cell death of SH-SY5Y cells

AU - Lee, Hak Ju

AU - Lyu, Da Hyun

AU - Koo, Uk

AU - Lee, Sung Jin

AU - Hong, Seong Su

AU - Kim, Kemok

AU - Kim, Kyeong Ho

AU - Lee, Dongho

AU - Mar, Woongchon

PY - 2011/8/1

Y1 - 2011/8/1

N2 - Three known 2-arylbenzofurans, moracin P (1), moracin O (2) and mulberrofuran Q (3) were isolated from the MeOH extract of the Mori Cortex Radicis. These compounds 1-3 enhanced cell viability in dose-dependent manner against oxygen-glucose deprivation (OGD)-induced cell death in neuroblastoma SH-SY5Y cells, which was measured by MTT reduction assay. (EC50 values of 10.4, 12.6, and 15.9 μM, respectively). In addition, the compounds 1-3 were examined for their inhibitory effect on OGD-induced ROS production by FACS analysis. We observed these compounds reduced ROS production in OGD-induced cell death (IC50 values of 1.9, 0.3 and 12.1 μM, respectively). Consequently, reactive oxygen species (ROS) were overexpressed in OGD-induced cells and all three compounds reduced ROS induced by OGD in dosedependent manner. Taken together, compounds 1-3 might protect neuronal cell death against the oxidative stress induced by OGD, though further studies in vitro and in vivo models are necessary.

AB - Three known 2-arylbenzofurans, moracin P (1), moracin O (2) and mulberrofuran Q (3) were isolated from the MeOH extract of the Mori Cortex Radicis. These compounds 1-3 enhanced cell viability in dose-dependent manner against oxygen-glucose deprivation (OGD)-induced cell death in neuroblastoma SH-SY5Y cells, which was measured by MTT reduction assay. (EC50 values of 10.4, 12.6, and 15.9 μM, respectively). In addition, the compounds 1-3 were examined for their inhibitory effect on OGD-induced ROS production by FACS analysis. We observed these compounds reduced ROS production in OGD-induced cell death (IC50 values of 1.9, 0.3 and 12.1 μM, respectively). Consequently, reactive oxygen species (ROS) were overexpressed in OGD-induced cells and all three compounds reduced ROS induced by OGD in dosedependent manner. Taken together, compounds 1-3 might protect neuronal cell death against the oxidative stress induced by OGD, though further studies in vitro and in vivo models are necessary.

KW - 2-Arylbenzofurans

KW - Moraceae

KW - Mori Cortex Radicis

KW - MTT

KW - Oxygen-glucose deprivation

KW - ROS

UR - http://www.scopus.com/inward/record.url?scp=80855140883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80855140883&partnerID=8YFLogxK

U2 - 10.1007/s12272-011-0818-4

DO - 10.1007/s12272-011-0818-4

M3 - Article

VL - 34

SP - 1373

EP - 1380

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

SN - 0253-6269

IS - 8

ER -