Inhibitory effect of arginine-derivatives from ginseng extract and basic amino acids on protein-arginine N-methyltransferase

B. C. Yoo, G. H. Park, H. Okuda, T. Takaku, S. Kim, Woo Ik Hwang

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    Protein-arginine N-methyltransferase (protein methylase I) catalyzes methylation of arginyl residues on substrate protein posttranslationally utilizing S-adenosyl-L-methionine as the methyl donor and yields N(G)-methylarginine residues. Arginyl-fructose and arginyl-fructosyl-glucose from Korean red ginseng were found to inhibit protein methylase I activity in vitro. This inhibitory activity was shown to be due to arginyl moiety in the molecules, rather than that of carbohydrates. Several basic amino acids as well as polyamines were also found to inhibit protein methylase I activity. Interestingly, the intensity of the inhibitory activity was correlated with the number of amino-group in polyamines, thus, in the order of spermine > spermidine > putrescine > agmatine-sulfate, with IC50 at approximately 15 mM, 25 mM, 35 mM, and 50 mM, respectively. On the other hand, neutral amino acids or NaCl did not inhibit the enzyme activity. Lineweaver-Burk plot analysis of the protein methylase I activity in the presence of arginine and spermidine indicated that the inhibition was competitive in nature in respect to protein substrate, with the K(i) values of 24.8 mM and 11.5 mM, respectively.

    Original languageEnglish
    Pages (from-to)391-400
    Number of pages10
    JournalAmino Acids
    Volume17
    Issue number4
    DOIs
    Publication statusPublished - 1999

    Keywords

    • Amino acids
    • Arginine derivatives
    • Basic amino acids
    • Ginseng extract
    • Inhibitors
    • Polyamines
    • Protein-arginine methyltransferase

    ASJC Scopus subject areas

    • Biochemistry
    • Clinical Biochemistry
    • Organic Chemistry

    Fingerprint

    Dive into the research topics of 'Inhibitory effect of arginine-derivatives from ginseng extract and basic amino acids on protein-arginine N-methyltransferase'. Together they form a unique fingerprint.

    Cite this