Inhibitory effect of L-type voltage-dependent calcium channel blocker on response of urinary bladder with acute ethanol intoxication

Mi Mi Oh, Jae Hyun Bae, Je Jong Kim, Hyung Jee Kim, Du Geon Moon, Jeong Gu Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: This study was performed to assess the effect of L-type voltage-dependent calcium channel (VDCC) blocker (dilitiazem) on the response of the urinary bladder with ethanol intoxication in in vivo and in vitro studies. Materials and Methods: Sprague-Dawley rats were used for in vivo and in vitro studies. The strips were divided into 5 groups according to pretreatment. Group I-A was treated with ethanol (0.1%), group I-B with ethanol (0.5%), group II with diltiazem treatment (10-6M), group III-A with pretreatment of diltiazem (10-6M) with ethanol intoxication (0.1%) and group III-B with pretreatment of diltiazem with ethanol intoxication (0.5%). The carbachol-induced tension was compared before and after each pretreatment. In separate in vivo experiments, the changes of maximal vesical pressure and intercontraction interval after intra-arterial administration of each agent (identical grouping with in vitro study) were monitored. Results: The carbachol-induced contractions in group I-A, group I-B, group II, group III-A and group III-B were significantly decreased after each pretreatment (95 ± 2.73%, 92.6 ± 2.5%, 65.4 ± 2.0%, 52.61 ± 5.16%, 14.9 ± 1.4% of the control). The degree of increment of intercontraction interval and decrement of maximal vesical pressure showed a significant difference in the presence of diltiazem and ethanol intoxication (0.5%) compared with the diltiazem-treated and ethanol-intoxicated groups (0.5%). Conclisions: There is a possibility that ethanol and L-type VDCC blockers have synergistic depressive effect on bladder contractility and that ethanol and L-type VDCC blockers act through a common ionic pathway.

Original languageEnglish
Pages (from-to)341-346
Number of pages6
JournalUrologia Internationalis
Volume85
Issue number3
DOIs
Publication statusPublished - 2010 Oct

Keywords

  • Bladder contractility
  • Calcium channel blocker
  • Ethanol intoxication
  • L-type voltage-dependent calcium channel blockers

ASJC Scopus subject areas

  • Urology

Fingerprint Dive into the research topics of 'Inhibitory effect of L-type voltage-dependent calcium channel blocker on response of urinary bladder with acute ethanol intoxication'. Together they form a unique fingerprint.

  • Cite this