Inhibitory effect of poncirus trifoliate on acetylcholinesterase and attenuating activity against trimethyltin-induced learning and memory impairment

Jae Kyeom Kim, Heyri Bae, Mi Jeong Kim, Soo Jung Choi, Hong Yon Cho, Han Joon Hwang, Young Jun Kim, Seung Taik Lim, Eun Ki Kim, Hye Kyung Kim, Bok Yong Kim, Dong Hoon Shin

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Various native Korean plants were screened to find an effective acetylcholinesterase (AChE) inhibitor for the treatment of Alzheimer's disease (AD). Among these plants, the ethanol extract of Poncirus trifoliate was selected for isolating the AChE inhibitor because it exhibited the highest inhibitory activity (47.31%). To separate the active compound from Poncirus trifoliate, solvent partition, open column chromatography, thinlayer chromatography (TLC), and high-performance liquid chromatography (HPLC) were utilized. The putative chemical structure of the AChE inhibitor was identified as methoxsalen by successive analysis with electron ionization mass spectrometry (EI-MS) and 13C/1H-nuclear magnetic resonance (NMR). To confirm the attenuating effect of the Poncirus trifoliate extract against trimethyltin (TMT)-induced neurotoxicity, in vivo behavior tests were carried out. Our findings suggest that the Poncirus trifoliate extract significantly reversed TMT-induced learning and memory impairment. These results demonstrate that the Poncirus trifoliate extract could possess a wide range of beneficial activities for neurodegenerative disorders, notably AD.

Original languageEnglish
Pages (from-to)1105-1112
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number5
DOIs
Publication statusPublished - 2009

Keywords

  • Acetylcholinesterase
  • Alzheimer's disease
  • Amyloid beta peptide
  • Methoxsalen (8-methoxy-20,30,6,7-furocoumarin)
  • Poncirus trifoliate

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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