Inhibitory effect of prostaglandin E2 on the migration of nasal fibroblasts

Jae Min Shin, Il Ho Park, You Mi Moon, Sung Moon Hong, Jung Sun Cho, Ji Young Um, Heung Man Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Fibroblast migration is crucial for normal wound repair after sinonasal surgery. Prostaglandin E2 (PGE2) is a potent inhibitor of fibroblast functions including chemotaxis, proliferation, and matrix production. The purpose of this study was to determine whether PGE 2 affects the migration of nasal fibroblasts and to investigate the mechanism of action of PGE2 on nasal fibroblasts. Methods: Primary cultures of nasal fibroblasts were established from inferior turbinate samples. Fibroblast migration was evaluated with scratch assays. Reverse-transcription polymerase chain reaction was performed for E prostanoid (EP) 1, EP2, EP3, and EP4 receptors. EP receptor-selective agonists and antagonists were used to evaluate receptor functions. Stimulatory G (Gs) proteins were activated to evaluate mechanisms. Intracellular cyclic adenosine monophosphate (cAMP) levels were measured by ELISA, and fibroblast cytoskeletal structures were visualized with immunocytochemistry. Results: PGE2 significantly reduced the migration of nasal fibroblasts. Agonists selective for the EP2 and EP4 receptors significantly reduced the nasal fibroblast migration. Antagonists of the EP2 and EP4 receptors inhibited the effect of PGE2 on nasal fibroblast migration. Activation of Gs protein and adenyl cyclase reduced nasal fibroblast migration. Conclusion: PGE2 inhibited the migration of nasal fibroblasts via the EP2 and EP4 receptors, and this inhibition was mediated by cAMP elevation. Targeting specific EP receptors could offer therapeutic opportunities for conditions such as delayed wound healing after nasal surgery.

Original languageEnglish
JournalAmerican Journal of Rhinology and Allergy
Volume28
Issue number3
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Nose
Dinoprostone
Fibroblasts
Prostaglandins
Cyclic AMP
Gs GTP-Binding Protein alpha Subunits
Nasal Surgical Procedures
Turbinates
Chemotaxis
Prostaglandins E
Adenylyl Cyclases
Wound Healing
Reverse Transcription
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Immunology and Allergy

Cite this

Inhibitory effect of prostaglandin E2 on the migration of nasal fibroblasts. / Shin, Jae Min; Park, Il Ho; Moon, You Mi; Hong, Sung Moon; Cho, Jung Sun; Um, Ji Young; Lee, Heung Man.

In: American Journal of Rhinology and Allergy, Vol. 28, No. 3, 01.01.2014.

Research output: Contribution to journalArticle

Shin, Jae Min ; Park, Il Ho ; Moon, You Mi ; Hong, Sung Moon ; Cho, Jung Sun ; Um, Ji Young ; Lee, Heung Man. / Inhibitory effect of prostaglandin E2 on the migration of nasal fibroblasts. In: American Journal of Rhinology and Allergy. 2014 ; Vol. 28, No. 3.
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abstract = "Background: Fibroblast migration is crucial for normal wound repair after sinonasal surgery. Prostaglandin E2 (PGE2) is a potent inhibitor of fibroblast functions including chemotaxis, proliferation, and matrix production. The purpose of this study was to determine whether PGE 2 affects the migration of nasal fibroblasts and to investigate the mechanism of action of PGE2 on nasal fibroblasts. Methods: Primary cultures of nasal fibroblasts were established from inferior turbinate samples. Fibroblast migration was evaluated with scratch assays. Reverse-transcription polymerase chain reaction was performed for E prostanoid (EP) 1, EP2, EP3, and EP4 receptors. EP receptor-selective agonists and antagonists were used to evaluate receptor functions. Stimulatory G (Gs) proteins were activated to evaluate mechanisms. Intracellular cyclic adenosine monophosphate (cAMP) levels were measured by ELISA, and fibroblast cytoskeletal structures were visualized with immunocytochemistry. Results: PGE2 significantly reduced the migration of nasal fibroblasts. Agonists selective for the EP2 and EP4 receptors significantly reduced the nasal fibroblast migration. Antagonists of the EP2 and EP4 receptors inhibited the effect of PGE2 on nasal fibroblast migration. Activation of Gs protein and adenyl cyclase reduced nasal fibroblast migration. Conclusion: PGE2 inhibited the migration of nasal fibroblasts via the EP2 and EP4 receptors, and this inhibition was mediated by cAMP elevation. Targeting specific EP receptors could offer therapeutic opportunities for conditions such as delayed wound healing after nasal surgery.",
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