Inhibitory effect of skatole (3-methylindole) on enterohemorrhagic Escherichia coli O157: H7 ATCC 43894 biofilm formation mediated by elevated endogenous oxidative stress

S. H. Choi, Y. Kim, S. Oh, S. Oh, Taehoon Chun, Sae Hun Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigated the effects of skatole (3-methylindole), which is one of the indole derivatives on the biofilm formation of EHEC O157:H7. Notably, skatole (100 μg ml-1) significantly reduced EHEC O157:H7 ATCC 43894 biofilm formation by 52% in 96-well polystyrene plates under quiescent conditions, with no effect on planktonic cell growth. The skatole sample was maintained in stable conditions for 24 h without degradation or evaporation via EHEC O157:H7 ATCC 43894. Importantly, skatole negatively triggered the expression of catalase in EHEC strains, as well as altered EHEC surface morphology. Our finding indicated that suppressed catalase activity via skatole might have been responsible for elevated endogenous oxidative stress and increment in oxidative metabolites might have led to damaged cell surfaces and a reduction in biofilm formation of EHEC O157:H7 ATCC 43894.

Original languageEnglish
Pages (from-to)454-461
Number of pages8
JournalLetters in Applied Microbiology
Volume58
Issue number5
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Skatole
Enterohemorrhagic Escherichia coli
Escherichia coli O157
Biofilms
Oxidative Stress
Catalase
Polystyrenes
Growth

Keywords

  • Biofilm
  • Catalase
  • EHEC O157:H7
  • Oxidative stress
  • Skatole

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

@article{29a3bdd583b34568ab8b91ba73539286,
title = "Inhibitory effect of skatole (3-methylindole) on enterohemorrhagic Escherichia coli O157: H7 ATCC 43894 biofilm formation mediated by elevated endogenous oxidative stress",
abstract = "We investigated the effects of skatole (3-methylindole), which is one of the indole derivatives on the biofilm formation of EHEC O157:H7. Notably, skatole (100 μg ml-1) significantly reduced EHEC O157:H7 ATCC 43894 biofilm formation by 52{\%} in 96-well polystyrene plates under quiescent conditions, with no effect on planktonic cell growth. The skatole sample was maintained in stable conditions for 24 h without degradation or evaporation via EHEC O157:H7 ATCC 43894. Importantly, skatole negatively triggered the expression of catalase in EHEC strains, as well as altered EHEC surface morphology. Our finding indicated that suppressed catalase activity via skatole might have been responsible for elevated endogenous oxidative stress and increment in oxidative metabolites might have led to damaged cell surfaces and a reduction in biofilm formation of EHEC O157:H7 ATCC 43894.",
keywords = "Biofilm, Catalase, EHEC O157:H7, Oxidative stress, Skatole",
author = "Choi, {S. H.} and Y. Kim and S. Oh and S. Oh and Taehoon Chun and Kim, {Sae Hun}",
year = "2014",
month = "1",
day = "1",
doi = "10.1111/lam.12212",
language = "English",
volume = "58",
pages = "454--461",
journal = "Letters in Applied Microbiology",
issn = "0266-8254",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Inhibitory effect of skatole (3-methylindole) on enterohemorrhagic Escherichia coli O157

T2 - H7 ATCC 43894 biofilm formation mediated by elevated endogenous oxidative stress

AU - Choi, S. H.

AU - Kim, Y.

AU - Oh, S.

AU - Oh, S.

AU - Chun, Taehoon

AU - Kim, Sae Hun

PY - 2014/1/1

Y1 - 2014/1/1

N2 - We investigated the effects of skatole (3-methylindole), which is one of the indole derivatives on the biofilm formation of EHEC O157:H7. Notably, skatole (100 μg ml-1) significantly reduced EHEC O157:H7 ATCC 43894 biofilm formation by 52% in 96-well polystyrene plates under quiescent conditions, with no effect on planktonic cell growth. The skatole sample was maintained in stable conditions for 24 h without degradation or evaporation via EHEC O157:H7 ATCC 43894. Importantly, skatole negatively triggered the expression of catalase in EHEC strains, as well as altered EHEC surface morphology. Our finding indicated that suppressed catalase activity via skatole might have been responsible for elevated endogenous oxidative stress and increment in oxidative metabolites might have led to damaged cell surfaces and a reduction in biofilm formation of EHEC O157:H7 ATCC 43894.

AB - We investigated the effects of skatole (3-methylindole), which is one of the indole derivatives on the biofilm formation of EHEC O157:H7. Notably, skatole (100 μg ml-1) significantly reduced EHEC O157:H7 ATCC 43894 biofilm formation by 52% in 96-well polystyrene plates under quiescent conditions, with no effect on planktonic cell growth. The skatole sample was maintained in stable conditions for 24 h without degradation or evaporation via EHEC O157:H7 ATCC 43894. Importantly, skatole negatively triggered the expression of catalase in EHEC strains, as well as altered EHEC surface morphology. Our finding indicated that suppressed catalase activity via skatole might have been responsible for elevated endogenous oxidative stress and increment in oxidative metabolites might have led to damaged cell surfaces and a reduction in biofilm formation of EHEC O157:H7 ATCC 43894.

KW - Biofilm

KW - Catalase

KW - EHEC O157:H7

KW - Oxidative stress

KW - Skatole

UR - http://www.scopus.com/inward/record.url?scp=84898649664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898649664&partnerID=8YFLogxK

U2 - 10.1111/lam.12212

DO - 10.1111/lam.12212

M3 - Article

C2 - 24372511

AN - SCOPUS:84898649664

VL - 58

SP - 454

EP - 461

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 5

ER -