Inhibitory effect of tributyltin on expression of steroidogenic enzymes in mouse testis

Suel Kee Kim, Jong-Hoon Kim, Jung Ho Han, Yong Dal Yoon

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Tributyltin (TBT) is known to disrupt the development of reproductive organs, thereby reducing fertility. The aim of this study was to evaluate the acute toxicity of TBT on the testicular development and steroid hormone production. Immature (3-week-old) male mice were given a single administration of 25, 50, or 100 mg/kg of TBT by oral gavage. Lumen formation in seminiferous tubule was remarkably delayed, and the number of apoptotic germ cells found inside the tubules was increased in the TBT-exposed animals, whereas no apoptotic signal was observed in interstitial Leydig cells. Reduced serum testosterone concentration and down-regulated expressions of the mRNAs for cholesterol side-chain cleavage enzyme (P450scc), 17α -hydroxylase/C17-20 lyase (P45017α), 3β -hydroxysteroid-dehydrogenase (3β -HSD), and 17β -hydroxysteroid-dehydrogenase (17β -HSD) were also observed after TBT exposure. Altogether, these findings demonstrate that exposure to TBT is associated with induced apoptosis of testicular germ cells and inhibition of steroidogenesis by reduction in the expression of steroidogenic enzymes in interstitial Leydig cells. These adverse effects of TBT would cause serious defects in testicular development and function.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalInternational Journal of Toxicology
Volume27
Issue number2
DOIs
Publication statusPublished - 2008 Mar 1

Fingerprint

Testis
Enzymes
Leydig Cells
Germ Cells
3-Hydroxysteroid Dehydrogenases
Steroid hormones
Cells
Cholesterol Side-Chain Cleavage Enzyme
Seminiferous Tubules
Lyases
Mixed Function Oxygenases
tributyltin
Fertility
Toxicity
Testosterone
Animals
Steroids
Hormones
Apoptosis
Messenger RNA

Keywords

  • Apoptosis
  • Steroidogenesis
  • Testis
  • Testosterone
  • Tributyltin

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Inhibitory effect of tributyltin on expression of steroidogenic enzymes in mouse testis. / Kim, Suel Kee; Kim, Jong-Hoon; Han, Jung Ho; Yoon, Yong Dal.

In: International Journal of Toxicology, Vol. 27, No. 2, 01.03.2008, p. 175-182.

Research output: Contribution to journalArticle

@article{36a73d703d5546dcba499d9d0b1dd77f,
title = "Inhibitory effect of tributyltin on expression of steroidogenic enzymes in mouse testis",
abstract = "Tributyltin (TBT) is known to disrupt the development of reproductive organs, thereby reducing fertility. The aim of this study was to evaluate the acute toxicity of TBT on the testicular development and steroid hormone production. Immature (3-week-old) male mice were given a single administration of 25, 50, or 100 mg/kg of TBT by oral gavage. Lumen formation in seminiferous tubule was remarkably delayed, and the number of apoptotic germ cells found inside the tubules was increased in the TBT-exposed animals, whereas no apoptotic signal was observed in interstitial Leydig cells. Reduced serum testosterone concentration and down-regulated expressions of the mRNAs for cholesterol side-chain cleavage enzyme (P450scc), 17α -hydroxylase/C17-20 lyase (P45017α), 3β -hydroxysteroid-dehydrogenase (3β -HSD), and 17β -hydroxysteroid-dehydrogenase (17β -HSD) were also observed after TBT exposure. Altogether, these findings demonstrate that exposure to TBT is associated with induced apoptosis of testicular germ cells and inhibition of steroidogenesis by reduction in the expression of steroidogenic enzymes in interstitial Leydig cells. These adverse effects of TBT would cause serious defects in testicular development and function.",
keywords = "Apoptosis, Steroidogenesis, Testis, Testosterone, Tributyltin",
author = "Kim, {Suel Kee} and Jong-Hoon Kim and Han, {Jung Ho} and Yoon, {Yong Dal}",
year = "2008",
month = "3",
day = "1",
doi = "10.1080/10915810801977906",
language = "English",
volume = "27",
pages = "175--182",
journal = "International Journal of Toxicology",
issn = "1091-5818",
publisher = "SAGE Publications Inc.",
number = "2",

}

TY - JOUR

T1 - Inhibitory effect of tributyltin on expression of steroidogenic enzymes in mouse testis

AU - Kim, Suel Kee

AU - Kim, Jong-Hoon

AU - Han, Jung Ho

AU - Yoon, Yong Dal

PY - 2008/3/1

Y1 - 2008/3/1

N2 - Tributyltin (TBT) is known to disrupt the development of reproductive organs, thereby reducing fertility. The aim of this study was to evaluate the acute toxicity of TBT on the testicular development and steroid hormone production. Immature (3-week-old) male mice were given a single administration of 25, 50, or 100 mg/kg of TBT by oral gavage. Lumen formation in seminiferous tubule was remarkably delayed, and the number of apoptotic germ cells found inside the tubules was increased in the TBT-exposed animals, whereas no apoptotic signal was observed in interstitial Leydig cells. Reduced serum testosterone concentration and down-regulated expressions of the mRNAs for cholesterol side-chain cleavage enzyme (P450scc), 17α -hydroxylase/C17-20 lyase (P45017α), 3β -hydroxysteroid-dehydrogenase (3β -HSD), and 17β -hydroxysteroid-dehydrogenase (17β -HSD) were also observed after TBT exposure. Altogether, these findings demonstrate that exposure to TBT is associated with induced apoptosis of testicular germ cells and inhibition of steroidogenesis by reduction in the expression of steroidogenic enzymes in interstitial Leydig cells. These adverse effects of TBT would cause serious defects in testicular development and function.

AB - Tributyltin (TBT) is known to disrupt the development of reproductive organs, thereby reducing fertility. The aim of this study was to evaluate the acute toxicity of TBT on the testicular development and steroid hormone production. Immature (3-week-old) male mice were given a single administration of 25, 50, or 100 mg/kg of TBT by oral gavage. Lumen formation in seminiferous tubule was remarkably delayed, and the number of apoptotic germ cells found inside the tubules was increased in the TBT-exposed animals, whereas no apoptotic signal was observed in interstitial Leydig cells. Reduced serum testosterone concentration and down-regulated expressions of the mRNAs for cholesterol side-chain cleavage enzyme (P450scc), 17α -hydroxylase/C17-20 lyase (P45017α), 3β -hydroxysteroid-dehydrogenase (3β -HSD), and 17β -hydroxysteroid-dehydrogenase (17β -HSD) were also observed after TBT exposure. Altogether, these findings demonstrate that exposure to TBT is associated with induced apoptosis of testicular germ cells and inhibition of steroidogenesis by reduction in the expression of steroidogenic enzymes in interstitial Leydig cells. These adverse effects of TBT would cause serious defects in testicular development and function.

KW - Apoptosis

KW - Steroidogenesis

KW - Testis

KW - Testosterone

KW - Tributyltin

UR - http://www.scopus.com/inward/record.url?scp=42049106290&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42049106290&partnerID=8YFLogxK

U2 - 10.1080/10915810801977906

DO - 10.1080/10915810801977906

M3 - Article

C2 - 18404541

AN - SCOPUS:42049106290

VL - 27

SP - 175

EP - 182

JO - International Journal of Toxicology

JF - International Journal of Toxicology

SN - 1091-5818

IS - 2

ER -