Inhibitory effect of yellow myrobalan (Terminalia chebula) extract on fibrosis induced by carbon tetrachloride in rat liver

Yun Chang Koo; Chung Oui Hong; Mi Hyun Nam; Ji Hoon Kim; Sung Yong Yang; Nam Hee Won; Tae Sun Park; Kwang Won Lee

doi:10.1007/s10068-013-0158-x

Inhibitory effect of yellow myrobalan (Terminalia chebula) extract on fibrosis induced by carbon tetrachloride in rat liver

Yun Chang Koo, Chung Oui Hong, Mi Hyun Nam, Ji Hoon Kim, Sung Yong Yang, Nam Hee Won, Tae Sun Park, Kwang Won Lee

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Ethyl acetate layer of methanol extract (TCE) of yellow myrobalan (Terminalia chebula) contains 2.4% of chebulic acid, a standard compound in TCE, which have a strong anti-oxidative effect and was reported in our previous study. Thirty-one male SD rats were randomly divided into 5 groups (normal control, CCl₄ control, TCE control, CCl₄+high dose TCE, and low dose TCE). Liver fibrosis was induced by i.p. injection of CCl₄ and TCE were orally administrated. TCE decreased the up-regulated malondealdehyde value and increased the down-regulated ratio of GSH/GSSG content and activities of GRd, GPx, and GST compared to the CCl₄ control group. Decreased dysfunction and inflammatory reaction in liver was confirmed by alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, relative liver weight and infiltration of neutrophils. Also, TCE inhibited phenotype change of hepatic stellate cells (HSC) by reducing α-smooth muscle actin (α-SMA) gene expression and protein production. Inhibition of collagen 1A1 gene expression and protein production were also confirmed. From these results, TCE may be a useful material for preventing liver fibrosis.

Original language	English
Pages (from-to)	871-880
Number of pages	10
Journal	Food Science and Biotechnology
Volume	22
Issue number	3
DOIs	https://doi.org/10.1007/s10068-013-0158-x
Publication status	Published - 2013 Jun

Keywords

Terminalia chebula
carbon tetrachloride
chebulic acid
liver fibrosis

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology

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10.1007/s10068-013-0158-x

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title = "Inhibitory effect of yellow myrobalan (Terminalia chebula) extract on fibrosis induced by carbon tetrachloride in rat liver",

abstract = "Ethyl acetate layer of methanol extract (TCE) of yellow myrobalan (Terminalia chebula) contains 2.4% of chebulic acid, a standard compound in TCE, which have a strong anti-oxidative effect and was reported in our previous study. Thirty-one male SD rats were randomly divided into 5 groups (normal control, CCl4 control, TCE control, CCl4+high dose TCE, and low dose TCE). Liver fibrosis was induced by i.p. injection of CCl4 and TCE were orally administrated. TCE decreased the up-regulated malondealdehyde value and increased the down-regulated ratio of GSH/GSSG content and activities of GRd, GPx, and GST compared to the CCl4 control group. Decreased dysfunction and inflammatory reaction in liver was confirmed by alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, relative liver weight and infiltration of neutrophils. Also, TCE inhibited phenotype change of hepatic stellate cells (HSC) by reducing α-smooth muscle actin (α-SMA) gene expression and protein production. Inhibition of collagen 1A1 gene expression and protein production were also confirmed. From these results, TCE may be a useful material for preventing liver fibrosis.",

keywords = "Terminalia chebula, carbon tetrachloride, chebulic acid, liver fibrosis",

author = "Koo, {Yun Chang} and Hong, {Chung Oui} and Nam, {Mi Hyun} and Kim, {Ji Hoon} and Yang, {Sung Yong} and Won, {Nam Hee} and Park, {Tae Sun} and Lee, {Kwang Won}",

note = "Funding Information: Acknowledgments This research was supported by a Korea University Grant. The authors thank the Korea University-CJ Food Safety Center for providing the equipment and facilities.",

year = "2013",

month = jun,

doi = "10.1007/s10068-013-0158-x",

language = "English",

volume = "22",

pages = "871--880",

journal = "Food Science and Biotechnology",

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publisher = "Springer Netherlands",

number = "3",

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T1 - Inhibitory effect of yellow myrobalan (Terminalia chebula) extract on fibrosis induced by carbon tetrachloride in rat liver

AU - Koo, Yun Chang

AU - Hong, Chung Oui

AU - Nam, Mi Hyun

AU - Kim, Ji Hoon

AU - Yang, Sung Yong

AU - Won, Nam Hee

AU - Park, Tae Sun

AU - Lee, Kwang Won

N1 - Funding Information: Acknowledgments This research was supported by a Korea University Grant. The authors thank the Korea University-CJ Food Safety Center for providing the equipment and facilities.

PY - 2013/6

Y1 - 2013/6

N2 - Ethyl acetate layer of methanol extract (TCE) of yellow myrobalan (Terminalia chebula) contains 2.4% of chebulic acid, a standard compound in TCE, which have a strong anti-oxidative effect and was reported in our previous study. Thirty-one male SD rats were randomly divided into 5 groups (normal control, CCl4 control, TCE control, CCl4+high dose TCE, and low dose TCE). Liver fibrosis was induced by i.p. injection of CCl4 and TCE were orally administrated. TCE decreased the up-regulated malondealdehyde value and increased the down-regulated ratio of GSH/GSSG content and activities of GRd, GPx, and GST compared to the CCl4 control group. Decreased dysfunction and inflammatory reaction in liver was confirmed by alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, relative liver weight and infiltration of neutrophils. Also, TCE inhibited phenotype change of hepatic stellate cells (HSC) by reducing α-smooth muscle actin (α-SMA) gene expression and protein production. Inhibition of collagen 1A1 gene expression and protein production were also confirmed. From these results, TCE may be a useful material for preventing liver fibrosis.

AB - Ethyl acetate layer of methanol extract (TCE) of yellow myrobalan (Terminalia chebula) contains 2.4% of chebulic acid, a standard compound in TCE, which have a strong anti-oxidative effect and was reported in our previous study. Thirty-one male SD rats were randomly divided into 5 groups (normal control, CCl4 control, TCE control, CCl4+high dose TCE, and low dose TCE). Liver fibrosis was induced by i.p. injection of CCl4 and TCE were orally administrated. TCE decreased the up-regulated malondealdehyde value and increased the down-regulated ratio of GSH/GSSG content and activities of GRd, GPx, and GST compared to the CCl4 control group. Decreased dysfunction and inflammatory reaction in liver was confirmed by alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, relative liver weight and infiltration of neutrophils. Also, TCE inhibited phenotype change of hepatic stellate cells (HSC) by reducing α-smooth muscle actin (α-SMA) gene expression and protein production. Inhibition of collagen 1A1 gene expression and protein production were also confirmed. From these results, TCE may be a useful material for preventing liver fibrosis.

KW - Terminalia chebula

KW - carbon tetrachloride

KW - chebulic acid

KW - liver fibrosis

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ER -

Inhibitory effect of yellow myrobalan (Terminalia chebula) extract on fibrosis induced by carbon tetrachloride in rat liver

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Keywords

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