Inhibitory effects of green tea catechin on the lipid accumulation in 3T3-l1 adipocytes

Mak Soon Lee, Chong Tai Kim, In Hwan Kim, Yangha Kim

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The aim of the present study was to evaluate the effects of green tea (-)-epigallocatechin-3-gallate (EGCG) on the depletion of accumulated fat in differentiated 3T3-L1 adipocytes. Intracellular lipid accumulation was decreased significantly after 24 h of incubation with 10 μM EGCG, while the viability of adipocytes was reported to be unaffected. Under the same experimental conditions, the amount of glycerol released from cells into the medium was increased by 10 μM EGCG. The level of mRNA in the 3T3-L1 adipocytes was analysed by quantitative real-time RT-PCR. EGCG notably increased the mRNA level of hormone sensitive lipase (HSL), which catalyses the rate-limiting stage in hydrolysis of stored triacylglycerol to monoacylglycerol and free fatty acids. In conclusion, the experiment produced results which showed that green tea EGCG effectively depleted fat accumulation via the stimulation of lipolysis and increased HSL gene expression in 3T3-L1 adipocytes. These results may relate to the mechanism by which EGCG modulates lipolysis in adipocytes.

Original languageEnglish
Pages (from-to)1088-1091
Number of pages4
JournalPhytotherapy Research
Volume23
Issue number8
DOIs
Publication statusPublished - 2009 Aug

Keywords

  • 3T3-l1 adipocytes
  • Green tea EGCG
  • Hormone sensitive lipase
  • Lipid

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Inhibitory effects of green tea catechin on the lipid accumulation in 3T3-l1 adipocytes'. Together they form a unique fingerprint.

  • Cite this