Inhibitory Effects of Pterocarpus santalinus Extract against IgE/Antigen-Sensitized Mast Cells and Atopic Dermatitis-Like Skin Lesions

Baknoon Ham, Myungsuk Kim, Yang Ju Son, Sungyul Chang, Sang Hoon Jung, Chu Won Nho, Man Jae Kwon

Research output: Contribution to journalArticle

Abstract

Pterocarpus santalinus has been traditionally used as a medicinal plant owing to its anti-inflammatory, anthelmintic, tonic, antihyperglycemic, and diaphoretic properties. We hypothesized that P. santalinus might have therapeutic potential in alleviating allergy and atopic dermatitis. Thus, we investigated the inhibitory effects of P. santalinus extract against allergic responses and 2,4-dinitrochlorobenzene-induced atopic dermatitis-like dorsal skin lesions using immunoglobulin E-sensitized rat basophilic leukemia-2H3 mast cells and NC/Nga mice. Degranulation and enzyme-linked immunosorbent assays were conducted to measure degranulation, proinflammatory cytokine levels, and prostaglandin E 2 concentrations in immunoglobulin E/antigen-sensitized RBL-2H3 mast cells. The therapeutic efficacy of P. santalinus extract in 2,4-dinitrochlorobenzene-induced atopic dermatitis was evaluated through morphological, physiological, and immunological analysis. P. santalinus extract inhibited β -hexosaminidase and histamine release and reduced tumor necrosis factor- α, interleukin-4, and prostaglandin E 2 secretion. Furthermore, P. santalinus extract suppressed atopic dermatitis-like skin lesions by regulating the serum levels of immunoglobulin E and immunoglobulin G2a, and messenger ribonucleic acid expression of T helper cell 1- and T helper cell 2-related mediators in the skin lesions. Histopathological analyses showed a decrease in epidermal thickness and intradermal inflammatory cell infiltration. These results suggested that P. santalinus extract might have beneficial effects in treating allergic and atopic dermatitis-like skin disorders.

Original languageEnglish
Pages (from-to)599-607
Number of pages9
JournalPlanta Medica
Volume85
Issue number7
DOIs
Publication statusPublished - 2019 Jan 1

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Atopic Dermatitis
Mast Cells
Immunoglobulin E
Skin
Antigens
Dinitrochlorobenzene
Prostaglandins E
Mast-Cell Leukemia
Pterocarpus
Allergies
Hexosaminidases
Immunosorbents
Th2 Cells
Th1 Cells
Anthelmintics
Histamine Release
Medicinal Plants
Infiltration
Hypoglycemic Agents
Interleukin-4

Keywords

  • atopic dermatitis
  • IgE
  • Leguminosae
  • Pterocarpus santalinus
  • RBL-2H3 mast cell
  • Th1/Th2-related mediators

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this

Inhibitory Effects of Pterocarpus santalinus Extract against IgE/Antigen-Sensitized Mast Cells and Atopic Dermatitis-Like Skin Lesions. / Ham, Baknoon; Kim, Myungsuk; Son, Yang Ju; Chang, Sungyul; Jung, Sang Hoon; Nho, Chu Won; Kwon, Man Jae.

In: Planta Medica, Vol. 85, No. 7, 01.01.2019, p. 599-607.

Research output: Contribution to journalArticle

Ham, Baknoon ; Kim, Myungsuk ; Son, Yang Ju ; Chang, Sungyul ; Jung, Sang Hoon ; Nho, Chu Won ; Kwon, Man Jae. / Inhibitory Effects of Pterocarpus santalinus Extract against IgE/Antigen-Sensitized Mast Cells and Atopic Dermatitis-Like Skin Lesions. In: Planta Medica. 2019 ; Vol. 85, No. 7. pp. 599-607.
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