Inhibitory effects of the κ-casein macropeptide isolated from milk protein on the biofilm formation and virulence of Listeria monocytogenes

Hyun Sun Yun, Younghoon Kim, Mi Ri Park, Sae Hun Kim, Sejong Oh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We demonstrate the inhibitory effects of κ-casein macropeptide (CMP) on the biofilm formation and virulence of Listeria monocytogenes Scott A. The inhibition of biofilm formation by CMP was initially investigated by using the protocol applied for the 96-well microtiter plate assay. Low concentrations of CMP (0.1, 0.2, 0.3, 0.4, and 0.5 mg/mL) that were tested resulted in a profound inhibitory effect on biofilm formation at a concentration of 0.4 mg/mL. CMP also significantly repressed the transcription of inlA (encoding internalin A) that was responsible for the initial adhesion and invasion event, and prolonged the survival of Caenorhabditis elegans infected by L. monocytogenes. Two-dimensional gel electrophoresis showed that newly identified proteins in the presence of CMP were involved in the stress response and metabolic processes that have important roles in developing listerial biofilms. Our results suggest that CMP from milk protein would be capable of eliminating biofilm formation and virulence by L. monocytogenes in the food industry.

Original languageEnglish
Pages (from-to)490-498
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Listeria
Milk Proteins
Listeria monocytogenes
Biofilms
Caseins
Virulence
Food Industry
Electrophoresis, Gel, Two-Dimensional
Caenorhabditis elegans
Transcription
Electrophoresis
Assays
Adhesion
Gels
Industry
Proteins

Keywords

  • Biofilm formation
  • Caenorhabditis elegans
  • Listeria monocytogenes
  • Two-dimensional gel electrophoresis
  • κ-casein macropeptide

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry
  • Medicine(all)

Cite this

Inhibitory effects of the κ-casein macropeptide isolated from milk protein on the biofilm formation and virulence of Listeria monocytogenes. / Yun, Hyun Sun; Kim, Younghoon; Park, Mi Ri; Kim, Sae Hun; Oh, Sejong.

In: Bioscience, Biotechnology and Biochemistry, Vol. 78, No. 3, 2014, p. 490-498.

Research output: Contribution to journalArticle

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