Abstract
We demonstrate the inhibitory effects of κ-casein macropeptide (CMP) on the biofilm formation and virulence of Listeria monocytogenes Scott A. The inhibition of biofilm formation by CMP was initially investigated by using the protocol applied for the 96-well microtiter plate assay. Low concentrations of CMP (0.1, 0.2, 0.3, 0.4, and 0.5 mg/mL) that were tested resulted in a profound inhibitory effect on biofilm formation at a concentration of 0.4 mg/mL. CMP also significantly repressed the transcription of inlA (encoding internalin A) that was responsible for the initial adhesion and invasion event, and prolonged the survival of Caenorhabditis elegans infected by L. monocytogenes. Two-dimensional gel electrophoresis showed that newly identified proteins in the presence of CMP were involved in the stress response and metabolic processes that have important roles in developing listerial biofilms. Our results suggest that CMP from milk protein would be capable of eliminating biofilm formation and virulence by L. monocytogenes in the food industry.
| Original language | English |
|---|---|
| Pages (from-to) | 490-498 |
| Number of pages | 9 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 78 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Biofilm formation
- Caenorhabditis elegans
- Listeria monocytogenes
- Two-dimensional gel electrophoresis
- κ-casein macropeptide
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Applied Microbiology and Biotechnology
- Analytical Chemistry
- Organic Chemistry
- Medicine(all)
Cite this
Inhibitory effects of the κ-casein macropeptide isolated from milk protein on the biofilm formation and virulence of Listeria monocytogenes. / Yun, Hyun Sun; Kim, Younghoon; Park, Mi Ri; Kim, Sae Hun; Oh, Sejong.
In: Bioscience, Biotechnology and Biochemistry, Vol. 78, No. 3, 2014, p. 490-498.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibitory effects of the κ-casein macropeptide isolated from milk protein on the biofilm formation and virulence of Listeria monocytogenes
AU - Yun, Hyun Sun
AU - Kim, Younghoon
AU - Park, Mi Ri
AU - Kim, Sae Hun
AU - Oh, Sejong
PY - 2014
Y1 - 2014
N2 - We demonstrate the inhibitory effects of κ-casein macropeptide (CMP) on the biofilm formation and virulence of Listeria monocytogenes Scott A. The inhibition of biofilm formation by CMP was initially investigated by using the protocol applied for the 96-well microtiter plate assay. Low concentrations of CMP (0.1, 0.2, 0.3, 0.4, and 0.5 mg/mL) that were tested resulted in a profound inhibitory effect on biofilm formation at a concentration of 0.4 mg/mL. CMP also significantly repressed the transcription of inlA (encoding internalin A) that was responsible for the initial adhesion and invasion event, and prolonged the survival of Caenorhabditis elegans infected by L. monocytogenes. Two-dimensional gel electrophoresis showed that newly identified proteins in the presence of CMP were involved in the stress response and metabolic processes that have important roles in developing listerial biofilms. Our results suggest that CMP from milk protein would be capable of eliminating biofilm formation and virulence by L. monocytogenes in the food industry.
AB - We demonstrate the inhibitory effects of κ-casein macropeptide (CMP) on the biofilm formation and virulence of Listeria monocytogenes Scott A. The inhibition of biofilm formation by CMP was initially investigated by using the protocol applied for the 96-well microtiter plate assay. Low concentrations of CMP (0.1, 0.2, 0.3, 0.4, and 0.5 mg/mL) that were tested resulted in a profound inhibitory effect on biofilm formation at a concentration of 0.4 mg/mL. CMP also significantly repressed the transcription of inlA (encoding internalin A) that was responsible for the initial adhesion and invasion event, and prolonged the survival of Caenorhabditis elegans infected by L. monocytogenes. Two-dimensional gel electrophoresis showed that newly identified proteins in the presence of CMP were involved in the stress response and metabolic processes that have important roles in developing listerial biofilms. Our results suggest that CMP from milk protein would be capable of eliminating biofilm formation and virulence by L. monocytogenes in the food industry.
KW - Biofilm formation
KW - Caenorhabditis elegans
KW - Listeria monocytogenes
KW - Two-dimensional gel electrophoresis
KW - κ-casein macropeptide
UR - http://www.scopus.com/inward/record.url?scp=84926083974&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84926083974&partnerID=8YFLogxK
U2 - 10.1080/09168451.2014.885829
DO - 10.1080/09168451.2014.885829
M3 - Article
C2 - 25036840
AN - SCOPUS:84926083974
VL - 78
SP - 490
EP - 498
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 3
ER -