Initial osteoblast-like cell response to pure titanium and zirconia/alumina ceramics

Hee Cheol Ko, Jung Suk Han, Maria Bächle, Jun Hyeog Jang, Sang-Wan Shin, Dae Joon Kim

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Objectives: Outstanding mechanical properties, resistance to scratching and high biocompatibility make zirconia/alumina ceramics interesting for dental applications. To solve the problem of the well-known low temperature degradation and to provide stable mechanical properties a novel zirconia alloy ((Y,Nb)-TZP/alumina) was developed. The aim of this study was to investigate the initial bone cell response to this new zirconia/alumina composite ceramic. Methods: HOS cells were cultured on zirconia/alumina composite (Zc) and pure titanium (Ti) discs. Surface topography was examined by atomic force microscopy (AFM), cell morphology by scanning electron microscopy (SEM). Cell proliferation (MTS) and alkaline phosphatase activity was measured at 1, 4 and 8 days. The mRNA expression of Cycline D1, the cell cycle regulating gene, integrin β 1, osteonectin (ON) and β-actin were evaluated by RT-PCR analysis after 12, 24 and 48 h. Results: Both substrates showed a very smooth character with Ra-values in the range of 0.002-0.113 μm supporting a continuous cellular growth. After 8 days, cell proliferation on Zc was higher than on Ti. The mRNA expression of cyclin D1 showed similar activity after 48 h on both surfaces, ALP activity was higher on Zc after 8 days. ON expression however showed no difference between the two groups. Significance: Our data demonstrate that this new zirconia composite ceramic showed at least equivalent or slightly better biological response of osteoblast-like HOS cells than pure titanium during a short-time cell culture period.

Original languageEnglish
Pages (from-to)1349-1355
Number of pages7
JournalDental Materials
Volume23
Issue number11
DOIs
Publication statusPublished - 2007 Nov 1

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Aluminum Oxide
Osteoblasts
Titanium
Zirconia
Alumina
Osteonectin
Composite materials
Cell proliferation
Zirconium alloys
Cell Proliferation
Mechanical properties
Messenger RNA
Cyclin D1
Phosphatases
cdc Genes
Surface topography
Biocompatibility
Atomic Force Microscopy
Cell culture
Integrins

Keywords

  • AFM
  • Cell proliferation
  • Osteoblast-like HOS cells
  • RT-PCR
  • SEM
  • Zirconia/alumina ceramic

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Initial osteoblast-like cell response to pure titanium and zirconia/alumina ceramics. / Ko, Hee Cheol; Han, Jung Suk; Bächle, Maria; Jang, Jun Hyeog; Shin, Sang-Wan; Kim, Dae Joon.

In: Dental Materials, Vol. 23, No. 11, 01.11.2007, p. 1349-1355.

Research output: Contribution to journalArticle

Ko, Hee Cheol ; Han, Jung Suk ; Bächle, Maria ; Jang, Jun Hyeog ; Shin, Sang-Wan ; Kim, Dae Joon. / Initial osteoblast-like cell response to pure titanium and zirconia/alumina ceramics. In: Dental Materials. 2007 ; Vol. 23, No. 11. pp. 1349-1355.
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