iNSC suppress macrophage-induced inflammation by repressing COX-2

Jin Hee Kim, Woong Sun, Dong Wook Han, Hong Joo Moon, Jangbo Lee

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Brain inflammation causes cell damage and death in diseases such as Alzheimer’s and Parkinson’s. In this study, we investigated whether early induced neural stem cells (iNSCs) could protect against cell death after treatment with THP1-derived macrophages. We developed an inflammatory model system with THP1-derived macrophages and cortical neuronal cells and investigated the therapeutic efficacy of iNSC against macrophage-induced inflammation in this model. Apoptosis was confirmed by double immunocytochemistry with NeuN and 4′,6-diamidino-2-phenylindole using terminal deoxynucleotidyl transferase-mediated digoxigenin-dUTP-biotin nick-end labeling. Cortical neuronal cells cultured with iNSCs exhibited fewer apoptotic cells than did cultures without iNSCs. The levels of inflammatory cytokines and vascular endothelial growth factor (VEGF) were analyzed by enzyme-linked immunosorbent assay. Cells cultured with iNSCs had lower levels of inflammatory cytokines and higher VEGF levels than those cultured without iNSCs. Western blot analysis for cyclooxygenase-2 (COX-2) showed a significantly lower level of COX-2 in cells cultured with iNSCs than in those cultured without iNSCs. Thus, early iNSCs administration reduced inflammation associated with neurological recovery, and this effect is mediated by COX-2 regulation. Our results suggest that iNSCs have potential therapeutic relevance, because they display strong anti-inflammatory functions that promote neuroprotection thorough the inflammatory response.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume51
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • Cyclooxygenase-2 (COX-2)
  • Induced neural stem cells (iNSCs)
  • Inflammatory

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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