Insights into eukaryotic multistep phosphorelay signal transduction revealed by the crystal structure of Ypd1p from Saccharomyces cerevisiae

Hyun Kyu Song, Jae Young Lee, Myong Gyong Lee, Jinho Moon, Kyeongsik Min, Jin Kuk Yang, Se Won Suh

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

'Two-component' phosphorelay signal transduction systems constitute a potential target for antibacterial and antifungal agents, since they are found exclusively in prokaryotes and lower eukaryotes (yeast, fungi, slime mold, and plants) but not in mammalian organisms. Saccharomyces cerevisiae Ypd1y, a key intermediate in the osmosensing multistep phosphorelay signal transduction, catalyzes the phosphoryl group transfer between response regulators. Its 1.8 Å structure, representing the first example of a eukaryotic phosphorelay protein, contains a four-helix bundle as in the HPt domain of Escherichia coli ArcB sensor kinase. However, Ypd1p has a 44-residue insertion between the last two helices of the helix bundle. The side-chain of His64, the site of phosphorylation, protrudes into the solvent. The structural resemblance between Ypd1p and ArcB HPt domain suggests that both prokaryotes and lower eukaryotes utilize the same basic protein fold for phosphorelay signal transduction. This study sheds light on the best characterized eukaryotic phosphorelay system.

Original languageEnglish
Pages (from-to)753-761
Number of pages9
JournalJournal of Molecular Biology
Volume293
Issue number4
DOIs
Publication statusPublished - 1999 Nov 5
Externally publishedYes

Fingerprint

Saccharomyces cerevisiae
Signal Transduction
Eukaryota
Fungi
Antifungal Agents
Proteins
Phosphotransferases
Yeasts
Phosphorylation
Escherichia coli
Anti-Bacterial Agents

Keywords

  • Crystal structure
  • Phosphorelay phosphotransferase
  • Saccharomyces cerevisiae
  • Signal transduction
  • Ypd1p

ASJC Scopus subject areas

  • Virology

Cite this

Insights into eukaryotic multistep phosphorelay signal transduction revealed by the crystal structure of Ypd1p from Saccharomyces cerevisiae. / Song, Hyun Kyu; Lee, Jae Young; Lee, Myong Gyong; Moon, Jinho; Min, Kyeongsik; Yang, Jin Kuk; Suh, Se Won.

In: Journal of Molecular Biology, Vol. 293, No. 4, 05.11.1999, p. 753-761.

Research output: Contribution to journalArticle

Song, Hyun Kyu ; Lee, Jae Young ; Lee, Myong Gyong ; Moon, Jinho ; Min, Kyeongsik ; Yang, Jin Kuk ; Suh, Se Won. / Insights into eukaryotic multistep phosphorelay signal transduction revealed by the crystal structure of Ypd1p from Saccharomyces cerevisiae. In: Journal of Molecular Biology. 1999 ; Vol. 293, No. 4. pp. 753-761.
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