Insights into new bacteriophages of Lactococcus garvieae belonging to the family Podoviridae

Seyed Mahdi Ghasemi, Majid Bouzari, Nima Shaykh Baygloo, Hyo-Ihl Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Lactococcus garvieae is an emerging pathogen responsible for lactococcosis, a serious disease in trout aquaculture. The identification of new bacteriophages against L. garvieae strains may be an effective way to fight this disease and to study the pathogen’s biology. Three L. garvieae phages, termed WP-1, WWP-2 and SP-2, were isolated from different environments, and their morphological features, genome restriction profiles and structural protein patterns were studied. Random cloning of HindIII-cut fragments was performed, and the fragments were partially sequenced for each phage. Although slight differences were observed by transmission electron microscopy, all of the phages had hexagonal heads and short non-contractile tails and were classified as members of the family Podoviridae. Restriction digestion analysis of the nucleic acids of the different phages revealed that the HindIII and AseI digests produced similar DNA fragment patterns. Additionally, SDS-PAGE analysis indicated that the isolated phages have similar structural proteins. The sequence BLAST results did not show any significant similarity with other previously identified phages. To the best of our knowledge, this study provides the first molecular characterization of L. garvieae phages.

Original languageEnglish
Pages (from-to)2909-2915
Number of pages7
JournalArchives of Virology
Volume159
Issue number11
DOIs
Publication statusPublished - 2014 Jan 1

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Podoviridae
Lactococcus
Bacteriophages
Pathogens
Aquaculture
Trout
Cloning
Transmission Electron Microscopy
Nucleic Acids
Tail
Organism Cloning
Polyacrylamide Gel Electrophoresis
Digestion
Proteins
Genes
Head
Genome

ASJC Scopus subject areas

  • Virology

Cite this

Insights into new bacteriophages of Lactococcus garvieae belonging to the family Podoviridae. / Ghasemi, Seyed Mahdi; Bouzari, Majid; Shaykh Baygloo, Nima; Chang, Hyo-Ihl.

In: Archives of Virology, Vol. 159, No. 11, 01.01.2014, p. 2909-2915.

Research output: Contribution to journalArticle

Ghasemi, Seyed Mahdi ; Bouzari, Majid ; Shaykh Baygloo, Nima ; Chang, Hyo-Ihl. / Insights into new bacteriophages of Lactococcus garvieae belonging to the family Podoviridae. In: Archives of Virology. 2014 ; Vol. 159, No. 11. pp. 2909-2915.
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