TY - JOUR
T1 - Insights into new bacteriophages of Lactococcus garvieae belonging to the family Podoviridae
AU - Ghasemi, Seyed Mahdi
AU - Bouzari, Majid
AU - Shaykh Baygloo, Nima
AU - Chang, Hyo Ihl
N1 - Funding Information:
This research was financially supported by the University of Isfahan and basic science research program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2012R1A1A2009084).
Publisher Copyright:
© 2014, Springer-Verlag Wien.
PY - 2014/10/17
Y1 - 2014/10/17
N2 - Lactococcus garvieae is an emerging pathogen responsible for lactococcosis, a serious disease in trout aquaculture. The identification of new bacteriophages against L. garvieae strains may be an effective way to fight this disease and to study the pathogen’s biology. Three L. garvieae phages, termed WP-1, WWP-2 and SP-2, were isolated from different environments, and their morphological features, genome restriction profiles and structural protein patterns were studied. Random cloning of HindIII-cut fragments was performed, and the fragments were partially sequenced for each phage. Although slight differences were observed by transmission electron microscopy, all of the phages had hexagonal heads and short non-contractile tails and were classified as members of the family Podoviridae. Restriction digestion analysis of the nucleic acids of the different phages revealed that the HindIII and AseI digests produced similar DNA fragment patterns. Additionally, SDS-PAGE analysis indicated that the isolated phages have similar structural proteins. The sequence BLAST results did not show any significant similarity with other previously identified phages. To the best of our knowledge, this study provides the first molecular characterization of L. garvieae phages.
AB - Lactococcus garvieae is an emerging pathogen responsible for lactococcosis, a serious disease in trout aquaculture. The identification of new bacteriophages against L. garvieae strains may be an effective way to fight this disease and to study the pathogen’s biology. Three L. garvieae phages, termed WP-1, WWP-2 and SP-2, were isolated from different environments, and their morphological features, genome restriction profiles and structural protein patterns were studied. Random cloning of HindIII-cut fragments was performed, and the fragments were partially sequenced for each phage. Although slight differences were observed by transmission electron microscopy, all of the phages had hexagonal heads and short non-contractile tails and were classified as members of the family Podoviridae. Restriction digestion analysis of the nucleic acids of the different phages revealed that the HindIII and AseI digests produced similar DNA fragment patterns. Additionally, SDS-PAGE analysis indicated that the isolated phages have similar structural proteins. The sequence BLAST results did not show any significant similarity with other previously identified phages. To the best of our knowledge, this study provides the first molecular characterization of L. garvieae phages.
UR - http://www.scopus.com/inward/record.url?scp=84919345044&partnerID=8YFLogxK
U2 - 10.1007/s00705-014-2142-z
DO - 10.1007/s00705-014-2142-z
M3 - Article
C2 - 24928734
AN - SCOPUS:84919345044
VL - 159
SP - 2909
EP - 2915
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 11
ER -