Abstract
Lactococcus garvieae is an emerging pathogen responsible for lactococcosis, a serious disease in trout aquaculture. The identification of new bacteriophages against L. garvieae strains may be an effective way to fight this disease and to study the pathogen’s biology. Three L. garvieae phages, termed WP-1, WWP-2 and SP-2, were isolated from different environments, and their morphological features, genome restriction profiles and structural protein patterns were studied. Random cloning of HindIII-cut fragments was performed, and the fragments were partially sequenced for each phage. Although slight differences were observed by transmission electron microscopy, all of the phages had hexagonal heads and short non-contractile tails and were classified as members of the family Podoviridae. Restriction digestion analysis of the nucleic acids of the different phages revealed that the HindIII and AseI digests produced similar DNA fragment patterns. Additionally, SDS-PAGE analysis indicated that the isolated phages have similar structural proteins. The sequence BLAST results did not show any significant similarity with other previously identified phages. To the best of our knowledge, this study provides the first molecular characterization of L. garvieae phages.
| Original language | English |
|---|---|
| Pages (from-to) | 2909-2915 |
| Number of pages | 7 |
| Journal | Archives of Virology |
| Volume | 159 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2014 Jan 1 |
Fingerprint
ASJC Scopus subject areas
- Virology
Cite this
Insights into new bacteriophages of Lactococcus garvieae belonging to the family Podoviridae. / Ghasemi, Seyed Mahdi; Bouzari, Majid; Shaykh Baygloo, Nima; Chang, Hyo-Ihl.
In: Archives of Virology, Vol. 159, No. 11, 01.01.2014, p. 2909-2915.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Insights into new bacteriophages of Lactococcus garvieae belonging to the family Podoviridae
AU - Ghasemi, Seyed Mahdi
AU - Bouzari, Majid
AU - Shaykh Baygloo, Nima
AU - Chang, Hyo-Ihl
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Lactococcus garvieae is an emerging pathogen responsible for lactococcosis, a serious disease in trout aquaculture. The identification of new bacteriophages against L. garvieae strains may be an effective way to fight this disease and to study the pathogen’s biology. Three L. garvieae phages, termed WP-1, WWP-2 and SP-2, were isolated from different environments, and their morphological features, genome restriction profiles and structural protein patterns were studied. Random cloning of HindIII-cut fragments was performed, and the fragments were partially sequenced for each phage. Although slight differences were observed by transmission electron microscopy, all of the phages had hexagonal heads and short non-contractile tails and were classified as members of the family Podoviridae. Restriction digestion analysis of the nucleic acids of the different phages revealed that the HindIII and AseI digests produced similar DNA fragment patterns. Additionally, SDS-PAGE analysis indicated that the isolated phages have similar structural proteins. The sequence BLAST results did not show any significant similarity with other previously identified phages. To the best of our knowledge, this study provides the first molecular characterization of L. garvieae phages.
AB - Lactococcus garvieae is an emerging pathogen responsible for lactococcosis, a serious disease in trout aquaculture. The identification of new bacteriophages against L. garvieae strains may be an effective way to fight this disease and to study the pathogen’s biology. Three L. garvieae phages, termed WP-1, WWP-2 and SP-2, were isolated from different environments, and their morphological features, genome restriction profiles and structural protein patterns were studied. Random cloning of HindIII-cut fragments was performed, and the fragments were partially sequenced for each phage. Although slight differences were observed by transmission electron microscopy, all of the phages had hexagonal heads and short non-contractile tails and were classified as members of the family Podoviridae. Restriction digestion analysis of the nucleic acids of the different phages revealed that the HindIII and AseI digests produced similar DNA fragment patterns. Additionally, SDS-PAGE analysis indicated that the isolated phages have similar structural proteins. The sequence BLAST results did not show any significant similarity with other previously identified phages. To the best of our knowledge, this study provides the first molecular characterization of L. garvieae phages.
UR - http://www.scopus.com/inward/record.url?scp=84919345044&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84919345044&partnerID=8YFLogxK
U2 - 10.1007/s00705-014-2142-z
DO - 10.1007/s00705-014-2142-z
M3 - Article
C2 - 24928734
AN - SCOPUS:84919345044
VL - 159
SP - 2909
EP - 2915
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 11
ER -