Insulin-like growth factor-I (IGF-I) protects myelination from undernutritional insult: Studies of transgenic mice overexpressing IGF-I in brain

Ping Ye, Kee Hyoung Lee, A. Joseph D'Ercole

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Using insulin-like growth factor-I (IGF-I)-overexpressing transgenic (Tg) mice as a model, we have shown that IGF-I promotes myelination by increasing the number of oligodendrocytes and stimulating the expression of myelin-specific protein genes. In the present study, we investigated whether IGF-I protects myelination from undernutritional insult in Tg mice. Mice were undernourished beginning from postnatal (P) day 1, a time coincident with the onset of transgene expression, and sacrificed at P20. Consistently with our previous studies, brain weights of undernourished non-Tg control mice were decreased by 18%. Brain weights of undernourished IGF-I Tg mice, however, were the same as those of well-fed control mice and much greater than those of undernourished control mice. The expression of two major myelin proteins [myelin basic protein (MBP) and proteolipid protein (PLP)] in cerebral cortex (CTX) and hippocampus (HIP) was decreased by 73-92% in undernourished control mice, as judged by Northern and Western blot hybridization. The abundances of MBP and PLP mRNAs and proteins, however, were decreased by only 40-70% in undernourished IGF-I Tg mice. To assess the number of oligodendrocytes and their precursors, antibodies specific for carbonic anhydrase II (CAII; an oligodendrocyte marker) and NG2 (a precursor marker) were used. Compared to their well-fed counterparts, undernourished control mice exhibited 17-30% decreases in the number of oligodendrocytes and their precursors in CTX and corpus callosum (CO), whereas well-fed IGF-I Tg mice had 21-35% increases in CTX and CC. Undernourished IGF-I Tg mice exhibited cell numbers similar to those of well-fed control mice. These data indicate that IGF-I protects myelination from undernutrition damage during development. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)700-708
Number of pages9
JournalJournal of Neuroscience Research
Volume62
Issue number5
DOIs
Publication statusPublished - 2000 Dec 1

Fingerprint

Insulin-Like Growth Factor I
Transgenic Mice
Oligodendroglia
Brain
Proteolipids
Myelin Proteins
Myelin Basic Protein
Carbonic Anhydrase II
Weights and Measures
Proteins
Corpus Callosum
Transgenes
Malnutrition
Northern Blotting
Cerebral Cortex
Hippocampus
Cell Count
Western Blotting
Messenger RNA
Antibodies

Keywords

  • IGF-I
  • MBP
  • Myelination
  • Oligodendrocyte
  • PLP
  • Precursors
  • Undernutrition

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Insulin-like growth factor-I (IGF-I) protects myelination from undernutritional insult : Studies of transgenic mice overexpressing IGF-I in brain. / Ye, Ping; Lee, Kee Hyoung; D'Ercole, A. Joseph.

In: Journal of Neuroscience Research, Vol. 62, No. 5, 01.12.2000, p. 700-708.

Research output: Contribution to journalArticle

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