Insulin microcrystals prepared by the seed zone method

Hyun Kyung Lee, Jai Hyun Kwon, Sang H. Park, Chan Wha Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A novel microcrystalliztion process for bovine insulin (<5 μm almost 90% in volume) was developed for pulmonary delivery using the seed zone method. The stability of bovine insulin microcrystals produced was investigated by the chromatographic analysis. The percentage of high molecular weight proteins and other insulin-related compounds of the insulin microcrystals were almost same compared with its raw material, while the percentage of A-21 desamido insulin was significantly low (P<0.001). The physicochemical properties of the bovine insulin microcrystals were measured using X-ray diffraction and differential scanning calorimetry. The physicochemical properties of the produced microcrystals were similar to those of the commercial insulin powder and milled crystalline form. Therefore, the bovine insulin microcrystals which were produced by the seed zone method maintain their physicochemical properties sufficiently good for the pulmonary delivery in terms of product-related impurities.

Original languageEnglish
Pages (from-to)447-451
Number of pages5
JournalJournal of Crystal Growth
Volume293
Issue number2
DOIs
Publication statusPublished - 2006 Aug 1

Fingerprint

insulin
Microcrystals
Insulin
microcrystals
Seed
seeds
delivery
Chromatographic analysis
Powders
Differential scanning calorimetry
Raw materials
Molecular weight
molecular weight
Impurities
heat measurement
Crystalline materials
X ray diffraction
proteins
Proteins
impurities

Keywords

  • A1. Microcrystal
  • A1. Pulmonary delivery
  • A1. Stability
  • A2. Seed zone method
  • B1. Insulin

ASJC Scopus subject areas

  • Condensed Matter Physics

Cite this

Insulin microcrystals prepared by the seed zone method. / Lee, Hyun Kyung; Kwon, Jai Hyun; Park, Sang H.; Kim, Chan Wha.

In: Journal of Crystal Growth, Vol. 293, No. 2, 01.08.2006, p. 447-451.

Research output: Contribution to journalArticle

Lee, Hyun Kyung ; Kwon, Jai Hyun ; Park, Sang H. ; Kim, Chan Wha. / Insulin microcrystals prepared by the seed zone method. In: Journal of Crystal Growth. 2006 ; Vol. 293, No. 2. pp. 447-451.
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